Direct Measurement of pH Profiles in Immobilized Enzyme Carriers during Kinetically Controlled Synthesis Using CLSM

Confocal laser scanning microscopy was applied to measure the pH value in the carrier of immobilized enzymes during the enzyme‐catalyzed synthesis. pH profiles with a high resolution are shown, with the pH increasing in the core of the particles. Significant differences occur for different carrier m...

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Bibliographic Details
Published in:Biotechnology progress 2001, Vol.17 (2), p.294-303
Main Authors: Spiess, Antje C., Kasche, Volker
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Calibration
cephalosporins
Chromatography, High Pressure Liquid
Enzymes, Immobilized - metabolism
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Immobilization of enzymes and other molecules
Immobilization techniques
Kinetics
Lasers
Methods. Procedures. Technologies
Microscopy, Confocal - methods
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Summary:Confocal laser scanning microscopy was applied to measure the pH value in the carrier of immobilized enzymes during the enzyme‐catalyzed synthesis. pH profiles with a high resolution are shown, with the pH increasing in the core of the particles. Significant differences occur for different carrier material, particle size, porosity and surface modification. The increased pH value is identified as one of the reasons leading to reduced enzyme selectivity in the penicillin amidase catalyzed synthesis of cephalosporins and penicillins.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp000149e