Neuroendocrine peptides stimulate adenyl cyclase in normal and malignant prostate cells

Elevations of intracellular cAMP in human prostate cancer cells have been shown to increase invasiveness and to promote neuronal differentiation. since neuroendocrine peptides capable of activating adenyl cyclase are present in prostatic nerves and epithelial neuroendocrine cells, we investigated no...

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Bibliographic Details
Published in:Regulatory peptides 1995-09, Vol.59 (1), p.43-51
Main Authors: Gkonos, Peter J., Lokeshwar, Balakrishna L., Balkan, Wayne, Roos, Bernard A.
Format: Article
Language:English
Subjects:
Adenylyl Cyclases - metabolism
Biological and medical sciences
Calcitonin
Calcitonin - pharmacology
Calcitonin gene-related peptide
Calcitonin Gene-Related Peptide - pharmacology
Cell Line
Enzyme Activation
Epithelial Cells - enzymology
Humans
Male
Medical sciences
Nephrology. Urinary tract diseases
Neuropeptides - pharmacology
Neurosecretory Systems - physiology
Prostate
Prostatic Neoplasms - enzymology
Receptor
Tumor Cells, Cultured
Tumors of the urinary system
Urinary tract. Prostate gland
Vasoactive intestinal peptide
Vasoactive Intestinal Peptide - pharmacology
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Summary:Elevations of intracellular cAMP in human prostate cancer cells have been shown to increase invasiveness and to promote neuronal differentiation. since neuroendocrine peptides capable of activating adenyl cyclase are present in prostatic nerves and epithelial neuroendocrine cells, we investigated normal and malignant human prostate cells for changes in intracellular cAMP in response to the prostatic peptides vasoactive intestinal peptide (VIP), calcitonin (CT), and calcitonin gene-related peptide (CGRP). Normal prostate epithelial cells and LNCaP prostate cancer cells exhibited, respectively, 6-and 30-fold increases in intracellular cAMP in response to VIP. ALVA-31 and PPC-1 prostate cancer cells demonstrated 20- to 200-fold increases in cAMP in response to CGRP, while normal epithelial cells and LNCaP cells exhibited smaller (2- to 6-fold) responses. Only DU-145 cells increased cAMP substantially in response to CT. VIP receptor mRNA was identified by Northern blot analysis only in those cells that responded to VIP. CT receptor mRNA was identified only in DU-145 cells by polymerase chain reaction and Southern blot analysis. These results suggest that VIP and possibly CGRP receptors are likely to be present in both normal and malignant prostate cells. VIP or CGRP may regulate secretion of proteases by normal or prostate cancer cells and may influence epithelial cell differentiation.
ISSN:0167-0115
1873-1686
DOI:10.1016/0167-0115(95)00072-J