Hydrogen peroxide preferentially enhances the tyrosine phosphorylation of epidermal growth factor receptor

We found that hydrogen peroxide (H 2O 2) enhances EGF receptor tyrosine phosphorylation in intact cells as well as solubilized membrane of an EGF receptor hyperproducing cell line NA. An antioxidant MnCl 2 effectively inhibited this enhancement. Interestingly, overall phosphorylation of the EGF rece...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1995-01, Vol.357 (2), p.161-164
Main Authors: Gamou, Shinobu, Shimizu, Nobuyoshi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antioxidants - pharmacology
Chlorides - pharmacology
DMEM
Dulbecco's modified Eagle medium
EGF
EGF receptor
epidermal growth factor
ErbB Receptors - metabolism
FBS
fetal bovine serum
Humans
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Manganese Compounds - pharmacology
Molecular Sequence Data
Oxidant stress
Peptide Mapping
Phosphorylation - drug effects
Protein phosphorylation
Trypsin
Tumor Cells, Cultured
Tyrosine - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We found that hydrogen peroxide (H 2O 2) enhances EGF receptor tyrosine phosphorylation in intact cells as well as solubilized membrane of an EGF receptor hyperproducing cell line NA. An antioxidant MnCl 2 effectively inhibited this enhancement. Interestingly, overall phosphorylation of the EGF receptor enhanced by H 2O 2 was half that of the EGF-enhanced phosphorylation when the receptor immunoprecipitated from [ 32P]ortho-phosphate-labeled cells was examined. Tryptic phospho-peptide mapping of these receptors revealed that EGF enhanced the phosphorylation on five specific residues including serine 671, 1,046 and 1,047, threonine 669 and tyrosine 1,173, whereas H 2O 2 enhanced the phosphorylation remarkably on tyrosine 1,173 and three other residues and only moderately on serine 1,046 and 1,047 and threonine 669. Thus, H 2O 2 preferentially enhances the tyrosine phosphorylation of EGF receptor through oxidant stress.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)01335-X