Nucleotide sequence of the structural genes for an anion pump. The plasmid-encoded arsenical resistance operon

The structural genes for the arsenical pump of the conjugative R-factor R773 contained on a HindIII fragment of 4.3 kilobase pairs were cloned into bacteriophage M13. A series of ordered deletions was created using Bal31 digestion, and the nucleotide sequence of the operon determined. Three open rea...

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Bibliographic Details
Published in:The Journal of biological chemistry 1986-11, Vol.261 (32), p.15030-15038
Main Authors: Chen, C M, Misra, T K, Silver, S, Rosen, B P
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - metabolism
Amino Acid Sequence
Arsenicals - pharmacology
Bacterial Proteins - genetics
Bacteriology
Base Sequence
Biological and medical sciences
Carrier Proteins - genetics
Conjugation, Genetic
Drug Resistance, Microbial
Escherichia coli - drug effects
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genetics
Microbiology
Models, Chemical
Operon
R Factors
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Summary:The structural genes for the arsenical pump of the conjugative R-factor R773 contained on a HindIII fragment of 4.3 kilobase pairs were cloned into bacteriophage M13. A series of ordered deletions was created using Bal31 digestion, and the nucleotide sequence of the operon determined. Three open reading frames for genes arsA, arsB, and arsC were found. The arsA gene encodes a hydrophilic protein of 63,169 Da with two potential adenylate-binding sites. The arsB gene encodes a potentially membrane protein of 45,577 Da. The arsC gene encodes a 15,811-Da hydrophilic protein. The arsA and arsC gene products correspond to cytosolic proteins previously identified from minicell experiments. Isolated ArsA protein was shown to bind to dye-agarose columns which act as affinity resins for nucleotide-binding proteins. A model is proposed in which these gene products form an anion translocating ATPase for extrusion of arsenite and arsenate from resistant cells.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)66824-3