Requirement of FGF-4 for Postimplantation Mouse Development

Fibroblast growth factors (FGFs) are thought to influence many processes in vertebrate development because of their diverse sites of expression and wide range of biological activities in in vitro culture systems. As a means of elucidating embryonic functions of FGF-4, gene targeting was used to gene...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1995-01, Vol.267 (5195), p.246-249
Main Authors: Feldman, Benjamin, Poueymirou, William, Papaioannou, Virginia E., DeChiara, Thomas M., Goldfarb, Mitchell
Format: Article
Language:English
Subjects:
Alleles
Analytical, structural and metabolic biochemistry
Animals
Base Sequence
Biological and medical sciences
Blastocyst
Blastocyst - cytology
Blastocyst - physiology
Cell growth
Cellular biology
Crosses, Genetic
Culture Techniques
Developmental biology
Developmental Stages
Embryo implantation
Embryology
Embryonic and Fetal Development - physiology
Embryonic Development - physiology
Embryos
Endometrium
Exons
Female
Fibroblast Growth Factor 4
Fibroblast growth factors
Fibroblast Growth Factors - genetics
Fibroblast Growth Factors - pharmacology
Fibroblast Growth Factors - physiology
Fundamental and applied biological sciences. Psychology
Gene Targeting
Genes
Genetic vectors
Genotypes
Growth
Heterozygote
Heterozygotes
House mouse
Individualized Instruction
Intermediary metabolites. Miscellaneous
Male
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Morula - drug effects
Morula - physiology
Other biological molecules
Phenotype
Pregnancy
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - pharmacology
Proto-Oncogene Proteins - physiology
Recombinant Proteins - pharmacology
Rodents
Zoology
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Description
Summary:Fibroblast growth factors (FGFs) are thought to influence many processes in vertebrate development because of their diverse sites of expression and wide range of biological activities in in vitro culture systems. As a means of elucidating embryonic functions of FGF-4, gene targeting was used to generate mice harboring a disrupted Fgf4 gene. Embryos homozygous for the null allele underwent uterine implantation and induced uterine decidualization but did not develop substantially thereafter. As was consistent with their behavior in vivo, Fgf4 null embryos cultured in vitro displayed severely impaired proliferation of the inner cell mass, whereas growth and differentiation of the inner cell mass were rescued when null embryos were cultured in the presence of FGF-4 protein.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7809630