Pathogenesis of IgA1 Protease-Producing and -Nonproducing Haemophilus influenzae in Human Nasopharyngeal Organ Cultures

We evaluated mucosal attachment, colonization, and invasion by Haemophilus influenzae in an experimental model of human nasopharyngeal tissue in organ culture. Non-piliated, encapsulated, and nonencapsulated, IgAl protease-deficient mutants of H. influenzae were compared with their isogenic IgAl pro...

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Bibliographic Details
Published in:The Journal of infectious diseases 1986-11, Vol.154 (5), p.752-759
Main Authors: Farley, M. M., Stephens, D. S., Mulks, M. H., Cooper, M. D., Bricker, J. V., Mirra, S. S., Wright, A.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Epithelial cells
Fundamental and applied biological sciences. Psychology
Haemophilus
Haemophilus Infections - enzymology
Haemophilus Infections - pathology
Haemophilus influenzae
Haemophilus influenzae - enzymology
Haemophilus influenzae - pathogenicity
Humans
Infections
Laboratory schools
Microbial colonization
Microbiology
Microscopy, Electron
Microscopy, Electron, Scanning
Mucosa
Nasopharynx - microbiology
Organ Culture Techniques
Original Articles
Pathogenesis
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Peptide Hydrolases - biosynthesis
Phagocytes
Serine Endopeptidases
Time Factors
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Summary:We evaluated mucosal attachment, colonization, and invasion by Haemophilus influenzae in an experimental model of human nasopharyngeal tissue in organ culture. Non-piliated, encapsulated, and nonencapsulated, IgAl protease-deficient mutants of H. influenzae were compared with their isogenic IgAl protease-producing parents. Damage to peripheral ciliary activity was first noted 6 hr after infection and was associated with sloughing of ciliated cells to which H. influenzae were not attached. Infection of organ cultures with each strain resulted in similar degrees and rates of ciliary damage. H. influenzae attached selectively to nonciliated epithelial cells or was associated with surface mucus. Later, disruption of epithelial tight junctions was observed, and clusters of H. influenzae were found between epithelial cells. Organisms were also seen within phagocytic vacuoles of mononuclear cells located above and below the basement membrane. In summary, encapsulated and nonencapsulated H. influenzae damaged the ciliary function of human nasopharyngeal organ cultures, attached to the mucosal surface, and invaded the epithelium. H. influenzae IgAl protease, however, was not essential for the pathogenic steps observed in this human nasopharyngeal organ culture model.
ISSN:0022-1899
1537-6613