Lack of a Requirement for the Fc Region of IgG in Restoring Pneumococcal Opsonization via the Alternative Complement Pathway in Sickle Cell Disease

Children with sickle cell disease have reduced serum opsonization of Streptococcus pneumoniae. Our previous studies have suggested that opsonization mediated by both the alternative and classic complement pathways is reduced because of a deficiency of IgG antibodies to pneumococcal capsular polysacc...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1986-11, Vol.154 (5), p.760-769
Main Authors: Bjornson, Ann B., Lobel, Jeffrey S.
Format: Article
Language:English
Subjects:
Adolescent
Alternative complement pathway
Anemia, Sickle Cell - immunology
Anemias. Hemoglobinopathies
Antibodies
Antiserum
Bacteria
Biological and medical sciences
Blood Bactericidal Activity
Child
Complement Activation
Complement C3 - analysis
Complement Pathway, Alternative
Diseases of red blood cells
Goats
Hematologic and hematopoietic diseases
Humans
Immunodiffusion
Immunoglobulin Fc Fragments - analysis
Immunoglobulin G - analysis
Immunoglobulins
Leukocytes
Medical sciences
Molecular Weight
Neutrophils - immunology
Opsonin Proteins
Original Articles
Phagocytosis
Polysaccharides
Sickle cell disease
Streptococcus pneumoniae - immunology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Children with sickle cell disease have reduced serum opsonization of Streptococcus pneumoniae. Our previous studies have suggested that opsonization mediated by both the alternative and classic complement pathways is reduced because of a deficiency of IgG antibodies to pneumococcal capsular polysaccharide. This study compares the ability of purified IgG (fractionated from goat antiserum to pneumococcal capsular polysaccharide) and F(ab′)2 fragments of the IgG preparation to restore alternative pathway-mediated opsonizaton of S. pneumoniae to sera from patients with sickle cell disease. Both the whole IgG preparation and F(ab′)2 fragments of this preparation restored opsonization to normal levels and concomitantly increased alternative pathway-mediated deposition of C3 onto the pneumococci to a supranormallevel. These results suggest that enhancement of opsonization is mediated by the F(ab′)2 region of IgG antibody to capsular polysaccharide and is associated with an increase in complement deposition on the bacterial surface.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/154.5.760