Schistosoma mansoni: Interaction of Nuclear Extracts with the CCAAT-Binding Site Revealed by the Gel Shift Assay

Analysis of the 5′-flanking region of the gene encoding the 28-kDa g]utathione S-transferase of Schistosoma mansoni (Sm28GST indicated the presence of motifs identical to AP-1 and CCAAT-family transcription factor recognition sequences. Gel retardation experiments showed that nuclear extracts from a...

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Bibliographic Details
Published in:Experimental parasitology 1995-02, Vol.80 (1), p.149-154
Main Authors: Zemzoumi, K., Dissous, C., Cochu, A., Trolet, J., Capron, A., Mcnair, A.
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
AMINO ACID SEQUENCES
Animals
Base Sequence
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Cell Nucleus - chemistry
CHEMICAL COMPOSITION
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Consensus Sequence
DNA
DNA SEQUENCING
DNA, Helminth - chemistry
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
genbank/l23117
Gene Expression Regulation
Genes, Helminth
GENETIC REGULATION
GENETICA
GENETICS
GENETIQUE
Invertebrates
Molecular Sequence Data
Nemathelminthia. Plathelmintha
NUCLEOTIDE SEQUENCE
Physiology. Development
Promoter Regions, Genetic
PROTEINAS
PROTEINE
PROTEINS
SCHISTOSOMA MANSONI
Schistosoma mansoni - chemistry
Schistosoma mansoni - genetics
SECUENCIA NUCLEICA
Sequence Alignment
SEQUENCE NUCLEIQUE
Transcription Factors - chemistry
Transcription Factors - genetics
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Summary:Analysis of the 5′-flanking region of the gene encoding the 28-kDa g]utathione S-transferase of Schistosoma mansoni (Sm28GST indicated the presence of motifs identical to AP-1 and CCAAT-family transcription factor recognition sequences. Gel retardation experiments showed that nuclear extracts from adult S. mansoni bound to an oligodeoxynucleotide containing at CCAAT box. A DNA fragment corresponding to the region of Sm28GST containing the CCAAT motif was demonstrated to interact with schistosome nuclear proteins. This binding was dependent on the presence of the CCAAT pentanucleotide motif. Nuclear factor Y (NF-Y) is a member of the CCAAT transcription factor family that has absolute requirement for the CCAAT sequence and that is highly conserved throughout evolution. The results of a PCR-based strategy aimed at cloning the NF-YA protein of S. mansoni are presented.
ISSN:0014-4894
1090-2449
DOI:10.1006/expr.1995.1017