Regulation of PLC-mediated signalling in vivo by CDP-diacylglycerol synthase

CDP-diacylglycerol synthase (CDS) is an enzyme required for the regeneration of the signaling molecule phosphatidylinositol-4,5-bisphosphate (PtdInsP2) from phosphatidic acid. A photoreceptor cell-specific isoform of CDS from Drosophila is a key regulator of phototransduction a G protein-coupled sig...

Full description

Saved in:
Bibliographic Details
Published in:Nature (London) 1995-01, Vol.373 (6511), p.216-222
Main Authors: Wu, L, Niemeyer, B, Colley, N, Socolich, M, Zuker, C.S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
CDPdiacylglycerol-Serine O-Phosphatidyltransferase - genetics
CDPdiacylglycerol-Serine O-Phosphatidyltransferase - metabolism
Cellular biology
compound eyes
Drosophila
Drosophila melanogaster
electroretinography
enzyme activity
Enzymes
GTP-Binding Proteins - metabolism
Humanities and Social Sciences
In Vitro Techniques
Insects
Light
Membranes
Molecular Sequence Data
multidisciplinary
mutants
Mutation
Nerve Degeneration
Neurology
Phosphatidic Acids - metabolism
Phosphatidylinositol 4,5-Diphosphate
Phosphatidylinositol Diacylglycerol-Lyase
Phosphatidylinositol Phosphates - metabolism
phosphatidylinositols
Phosphoric Diester Hydrolases - metabolism
Photoreceptor Cells, Invertebrate - metabolism
Photoreceptor Cells, Invertebrate - ultrastructure
photoreceptors
regulation
Science
Science (multidisciplinary)
Signal Transduction
transferases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:CDP-diacylglycerol synthase (CDS) is an enzyme required for the regeneration of the signaling molecule phosphatidylinositol-4,5-bisphosphate (PtdInsP2) from phosphatidic acid. A photoreceptor cell-specific isoform of CDS from Drosophila is a key regulator of phototransduction a G protein-coupled signaling cascade mediated by phospholipase C. cds mutants cannot sustain a light-activated current as a result of depletion of PtdInsP2. Overexpression of CDS increases the amplitude of the light response, demonstrating that availability of PtdInsP2 is a determinant in the gain of this pathway. cds mutants undergo light-dependent retinal degeneration which can be suppressed by a mutation in phospholipase C. Thus, enzymes involved in PtdInsP2 metabolism regulate phosphoinositide-mediated signaling cascades in vivo.
ISSN:0028-0836
1476-4687
DOI:10.1038/373216a0