Intron-exon structure of the porcine IκBα-encoding gene

Genomic clones of ECI-6 (endothelial cell inducible), the porcine IκBα gene that encodes a cytoplasmic inhibitor of the transcription factor NF-κB, were isolated, spanning the entire transcribed region plus 2.1 and 0.35 kb of 5′- and 3′-flanking sequences, respectively. The gene contains five intron...

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Bibliographic Details
Published in:Gene 1995-01, Vol.152 (2), p.253-255
Main Authors: de Martin, Rainer, Holzmüller, Harry, Hofer, Erhard, Bach, Fritz H.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
ankyrin
Base Sequence
Blotting, Southern
DNA, Complementary
DNA-Binding Proteins - antagonists & inhibitors
DNA-Binding Proteins - genetics
ECI-6
Exons
Genome
genomic organization
I-kappa B Proteins
Introns
Molecular Sequence Data
NF-KappaB Inhibitor alpha
NF-κB inhibitor
Swine
transcription factor
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Summary:Genomic clones of ECI-6 (endothelial cell inducible), the porcine IκBα gene that encodes a cytoplasmic inhibitor of the transcription factor NF-κB, were isolated, spanning the entire transcribed region plus 2.1 and 0.35 kb of 5′- and 3′-flanking sequences, respectively. The gene contains five introns ranging in size from 0.6 to 0.1 kb. Four of the introns are located in the coding regions for four of the five ankyrin-like repeats in the central part of the IκBα protein at similar positions. The fifth intron is located in the C-terminal region. Southern blot analysis indicates the presence of a single copy of ECI-6/IκBα in the porcine genome.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(94)00726-9