Pulsed-Field Gel Electrophoresis for Genomic Analysis of Neisseria gonorrhoeae

Pulsed-field gel electrophoresis (PFGE) is a technique for analyzing large DNA fragments. PFGE was compared to auxotyping and Por serovar (A/S) classification to determine its utility in the study of gonococcal infection, subtyping A/S classes, and clone identification. PFGE patterns were stable in...

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Bibliographic Details
Published in:The Journal of infectious diseases 1995-02, Vol.171 (2), p.455-458
Main Authors: Xia, Minsheng, Whittington, William L., Holmes, King K., Plummer, Francis A., Roberts, Marilyn C.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Concise Communication
Contact Tracing
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA
DNA, Bacterial - classification
DNA, Bacterial - metabolism
Electrophoresis
Electrophoresis, Gel, Pulsed-Field
Enzymes
Epidemiologic studies
Epidemiology
Female
Fundamental and applied biological sciences. Psychology
Gels
Genotype
Gonorrhea
Gonorrhea - microbiology
Humans
Infections
Male
Microbiology
Neisseria gonorrhoeae
Neisseria gonorrhoeae - classification
Neisseria gonorrhoeae - genetics
Plasmids
Sexual Partners
Systematics
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Description
Summary:Pulsed-field gel electrophoresis (PFGE) is a technique for analyzing large DNA fragments. PFGE was compared to auxotyping and Por serovar (A/S) classification to determine its utility in the study of gonococcal infection, subtyping A/S classes, and clone identification. PFGE patterns were stable in vitro after 2 isolates were passaged 50 times, and those from sex partners were indistinguishable with both enzymes. Fourteen proline-requiring IA-6 isolates from Kenya produced 8 NheI and 7 XbaI patterns; these included 6 Tet M-carrying isolates, all producing the same NheI pattern. PFGE patterns of 14 arginine-, hypoxanthine-, and uracil-requiring IA-l,2 isolates, from the US Pacific Northwest and Hawaii in 1993, were less diverse with both enzymes. PFGE analysis represents a potentially useful addition to the current gonococcal classification system.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/171.2.455