A 19-kDa Protein Belonging to a New Family Is Expressed in the Golgi Apparatus of Neural Cells (∗)

The mouse 8.5 mRNA encodes a 171-residue novel protein which displays a highly significant similarity with the product of the previously characterized neuronal p1A75 cDNA (Sutcliffe, J. G., Milner, R. J., Shinnick, T. M., and Bloom, F. E.(1983) Cell 33, 671-682). Northern blot and in situ hybridizat...

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Bibliographic Details
Published in:The Journal of biological chemistry 1995-01, Vol.270 (4), p.1888-1893
Main Authors: Sabéran-Djoneidi, Délara, Marey-Semper, Isabel, Picart, Renée, Studler, Jeanne-Marie, Tougard, Claude, Glowinski, Jacques, Lévi-Strauss, Matthieu
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Brain - metabolism
Cell Line
Chlorocebus aethiops
DNA Primers
Epidermal Growth Factor - chemistry
Fluorescent Antibody Technique
Gene Expression
Gene Library
Golgi Apparatus - metabolism
In Situ Hybridization
Kidney
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Molecular Weight
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - chemistry
Neurons - metabolism
Open Reading Frames
Protein Biosynthesis
Protein Conformation
RNA, Messenger - biosynthesis
Sequence Homology, Amino Acid
Transfection
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Summary:The mouse 8.5 mRNA encodes a 171-residue novel protein which displays a highly significant similarity with the product of the previously characterized neuronal p1A75 cDNA (Sutcliffe, J. G., Milner, R. J., Shinnick, T. M., and Bloom, F. E.(1983) Cell 33, 671-682). Northern blot and in situ hybridization experiments indicated that the 8.5 mRNA is specifically expressed in neural and neuroendocrine tissues. An affinity-purified antibody directed against the recombinant 8.5 protein demonstrated the existence of the 19-kDa natural protein in brain and evidenced its prominent juxtanuclear Golgi-like localization in cultured neurons. Ultrastructural analysis of the same preparation revealed a specific labeling of all the Golgi saccules and of some vesicles in the Golgi zone. In transfected COS cells, the exogenous protein was also detected in the Golgi area, indicating, therefore, the presence of a Golgi targeting signal in its primary sequence.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.270.4.1888