Retention of phagocytic functions in cryopreserved human monocytes

Phagocytosis and respiratory burst activity were measured by flow cytometry in fresh and cryopreserved human monocytes, after ingestion of Escherichia coli and Staphylococcus aureus. Mononuclear leukocytes, isolated from 15 healthy donors, were divided into two portions, of which one was examined im...

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Bibliographic Details
Published in:Journal of leukocyte biology 1995-02, Vol.57 (2), p.235-241
Main Authors: Hansen, John‐Bjarne, Halvorsen, Dag Seeger, Haldorsen, Bjørg Christina, Olsen, Randi, Sjursen, Haakon, Kierulf, Peter
Format: Article
Language:English
Subjects:
Adult
Blood Preservation
Cryopreservation
Escherichia coli
Female
Flow Cytometry
Humans
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - metabolism
Leukocytes, Mononuclear - physiology
Lymphocytes - cytology
Male
Microscopy, Electron
Middle Aged
monocytes
Phagocytosis
Respiratory Burst
respiratory burst activity
Staphylococcus aureus
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Summary:Phagocytosis and respiratory burst activity were measured by flow cytometry in fresh and cryopreserved human monocytes, after ingestion of Escherichia coli and Staphylococcus aureus. Mononuclear leukocytes, isolated from 15 healthy donors, were divided into two portions, of which one was examined immediately and the other was cryopreserved for 3 weeks. Morphological characteristics and expression of receptors involved in phagocytosis were similar in fresh and cryopreserved monocytes. Furthermore, both internalization of bacteria and respiratory burst activity remained unchanged after cryopreservation. Transmission electron microscopy confirmed actual internalization of bacteria and not merely bacterial attachment to monocytes. Monocytes were demonstrated to retain integral cellular functions during cryopreservation. This may imply that the method has potential for use in basal and clinical trials. J. Leukoc. Biol. 57: 235–241; 1995.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.57.2.235