Chromosomal Localization of Muscle Nicotinic Acetylcholine Receptor Genes in the Mouse

• Published in: Science (American Association for the Advancement of Science) 1986-11, Vol.234 (4778), p.866-868
• Main Authors: Heidmann, Odile, Buonanno, Andrés, Geoffroy, Brigitte, Robert, Benoît, Guénet, Jean-Louis, Merlie, John P., Changeux, Jean-Pierre
• Format: Article
• Language: English
• Subjects: Acetylcholine; Actins; Actins - genetics; Animals; Biochemistry; Biological and medical sciences; Blood vessels; Cell receptors; Chromosome Mapping; Chromosomes; Classical genetics, quantitative genetics, hybrids; Complementary DNA; Crosses, Genetic; DNA - genetics; DNA probes; DNA Restriction Enzymes; Fundamental and applied biological sciences. Psychology; Genes; Genetic aspects; Genetic loci; Genetic research; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Innovations; Mice; Mice, Inbred DBA; Muridae; Muscles - analysis; Neurons; Nuclear membrane; Nucleic Acid Hybridization; Polymorphism, Genetic; Receptors, Nicotinic - genetics; Rodents; Species Specificity; Vertebrata
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.3022377

The chromosomal localization of the genes encoding the four subunits of muscle nicotinic receptor was determined by analyzing restriction fragment length polymorphisms between two mouse species Mus musculus domesticus (DBA/2) and Mus spretus (SPE). Analysis of the progeny of the interspecies mouse backcross (DBA/2 $\times $ SPE) $\times $ DBA/2 showed that the $\alpha $-subunit gene cosegregates with the $\alpha $-cardiac actin gene on chromosome 17, that the $\beta $-subunit gene is located on chromosome 11, and that the $\gamma $- and $\delta $-subunit genes cosegregate and are located on chromosome 1.