Occurrence of Novel Types of Nitric Oxide Synthase in the Silkworm, Bombyx mori

Nitric oxide (NO) synthase activity was detected in fat body and the Malpighian tubles of the silkworm, Bombyx mori. Main NO synthase activity in the fat body was Ca 2+/calmodulin-dependent, inducible by bacterial lipopolysaccharide (LPS) and required NADPH, FAD, FMN, dithiothreitol (DTT) and tetrah...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-02, Vol.207 (1), p.452-459
Main Authors: Choi, S.K., Choi, H.K., Kadonookuda, K., Taniai, K., Kato, Y., Yamamoto, M., Chowdhury, S., Xu, J.H., Miyanoshita, A., Debnath, N.C., Asaoka, A., Yamakawa, M.
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Amino Acid Oxidoreductases - analysis
Amino Acid Oxidoreductases - biosynthesis
Amino Acid Oxidoreductases - metabolism
Animals
APPAREIL DIGESTIF
Bombyx - enzymology
BOMBYX MORI
Calmodulin - antagonists & inhibitors
Calmodulin - pharmacology
Dose-Response Relationship, Drug
ENZIMAS
ENZYME
Enzyme Induction
Fat Body - enzymology
INHIBICION
INHIBITION
Isoenzymes - analysis
Isoenzymes - biosynthesis
Isoenzymes - metabolism
Kinetics
LIPIDE
LIPIDOS
Lipopolysaccharides - pharmacology
Malpighian Tubules - enzymology
Nitric Oxide Synthase
OXIDOS DE NITROGENO
OXYDE D'AZOTE
SISTEMA DIGESTIVO
SISTEMA UROGENITAL
Sulfonamides - pharmacology
SYSTEME UROGENITAL
Trifluoperazine - pharmacology
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Summary:Nitric oxide (NO) synthase activity was detected in fat body and the Malpighian tubles of the silkworm, Bombyx mori. Main NO synthase activity in the fat body was Ca 2+/calmodulin-dependent, inducible by bacterial lipopolysaccharide (LPS) and required NADPH, FAD, FMN, dithiothreitol (DTT) and tetrahydrobiopterin (BH 4) as cofactors for the full expression of the activity. The Malpighian tubles contained two types of NO synthase. One was Ca 2+-independent, calmodulin-dependent and constitutive and the other was Ca 2+-dependent and constitutive. The former NO synthase required the same cofactors as fat body NO synthase. The activity of Malpighian tuble NO synthases increased dramatically at the end of the last instar period, just prior to spinning. These results indicate that B. mori contains new types of NO synthase, suggesting the wide distribution and different characteristics of this enzyme among vertebrates and invertebrates.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.1209