Determination of doubly labeled water by gas-phase fourier transform infrared spectroscopy

Both 2H (deuterium) and 18O (oxygen 18) in isotopically enriched water have been detected by gas-phase Fourier transform infrared (FTIR) spectroscopy at 2,720 and 3,661.8 cm −1, respectively. A linear relationship between varying concentrations of each of these isotopes and their absorbance at the a...

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Bibliographic Details
Published in:Metabolism, clinical and experimental clinical and experimental, 1995, Vol.44 (1), p.1-3
Main Authors: Khaled, M.A., Krumdieck, C.L., Ong, J.L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Body Water - metabolism
Deuterium
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Metabolic diseases
Osmolar Concentration
Oxygen Isotopes
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Regression Analysis
Spectroscopy, Fourier Transform Infrared - methods
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Summary:Both 2H (deuterium) and 18O (oxygen 18) in isotopically enriched water have been detected by gas-phase Fourier transform infrared (FTIR) spectroscopy at 2,720 and 3,661.8 cm −1, respectively. A linear relationship between varying concentrations of each of these isotopes and their absorbance at the above frequencies indicates that gas-phase FTIR may provide a rapid and potentially less expensive approach to measure doubly labeled water in biological fluids for the estimation of energy expenditure and total body water.
ISSN:0026-0495
1532-8600
DOI:10.1016/0026-0495(95)90279-1