Human gliomas with wild-type p53 express bcl-2

Human astrocytomas frequently overexpress wild-type p53, which suggests that gliomas have evolved a mechanism to subvert p53-mediated apoptosis. bcl-2 inhibits apoptosis mediated by p53, and it is expressed in several human cancers. We therefore examined a series of human gliomas to determine whethe...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1995-03, Vol.55 (5), p.999-1001
Main Authors: ALDERSON, L. M, CASTLEBERG, R. L, HARSH, G. R, LOUIS, D. N, HENSON, J. W
Format: Article
Language:English
Subjects:
apoptosis
Apoptosis - physiology
Astrocytoma - chemistry
Astrocytoma - genetics
Astrocytoma - pathology
bcl-2 gene
Biological and medical sciences
Gene Expression
Genes, p53 - genetics
glioma
Glioma - chemistry
Glioma - genetics
Glioma - pathology
Immunohistochemistry
man
Medical sciences
Mutation
Neurology
p53 gene
Paraffin Embedding
Proto-Oncogene Proteins - analysis
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-bcl-2
Tumor Suppressor Protein p53 - analysis
Tumor Suppressor Protein p53 - genetics
Tumors of the nervous system. Phacomatoses
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Summary:Human astrocytomas frequently overexpress wild-type p53, which suggests that gliomas have evolved a mechanism to subvert p53-mediated apoptosis. bcl-2 inhibits apoptosis mediated by p53, and it is expressed in several human cancers. We therefore examined a series of human gliomas to determine whether bcl-2 is expressed and whether this expression is associated with tumors which have wild-type p53. Twenty-eight paraffin-embedded gliomas (3 WHO grade II, 13 grade III, 12 grade IV) were immunohistochemically stained for bcl-2 and p53. p53 mutations were identified with single strand conformation polymorphism and DNA sequencing. Sixteen of 28 (57%) tumors expressed bcl-2, and bcl-2 expression was associated with wild-type p53 (P < 0.01). Among gliomas which overexpressed p53, bcl-2 was positive in 7 of 7 tumors with wild-type p53 but in only 1 of 7 with mutant p53 (P < 0.01). We conclude that bcl-2 is frequently expressed in human gliomas and that expression is more common in tumors with wild-type p53.
ISSN:0008-5472
1538-7445