Pulsed-field gel electrophoresis fingerprinting for identification of Azospirillum species

Pulsed-field gel electrophoresis (PFGE) was used to obtain macrorestriction fingerprints of restriction enzyme-cut DNA of natural isolates of Azospirillum spp. Metabolic profiles, along with other phenotypic characteristics, were compared with these fingerprints to differentiate among the azospirill...

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Bibliographic Details
Published in:Current microbiology 1995-03, Vol.30 (3), p.127-131
Main Authors: Eid, M.M.A. (Montana State University, Bozeman, MT.), Sherwood, J.E
Format: Article
Language:English
Subjects:
ADN
AZOSPIRILLUM
Azospirillum - classification
Azospirillum - cytology
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Sequence
Biological and medical sciences
Biotechnology
DNA Fingerprinting - methods
DNA, Bacterial - genetics
ELECTROFORESIS
ELECTROPHORESE
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
IDENTIFICACION
IDENTIFICATION
Microbiology
Molecular Sequence Data
RHIZOSPHERE
RIZOSFERA
Species Specificity
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Summary:Pulsed-field gel electrophoresis (PFGE) was used to obtain macrorestriction fingerprints of restriction enzyme-cut DNA of natural isolates of Azospirillum spp. Metabolic profiles, along with other phenotypic characteristics, were compared with these fingerprints to differentiate among the azospirilla isolates. A wide diversity of phenotypes (e.g., colony color, motility, and accumulation of poly-beta-hydroxybutyrate granules) was observed among the natural isolates of azospirilla. PFGE revealed that TCTAGA, the sequence recognized by Xba1, is rare in the genome of azospirilla. The PFGE fingerprint revealed that azospirilla associated with different crops have a very similar genetic background. PFGE fingerprints were more consistent in the identification of azospirilla isolates from specific hosts than the metabolic fingerprints. For further differentiation at strain level, metabolic, physiological, and morphological profiles provide additional information
ISSN:0343-8651
1432-0991
DOI:10.1007/BF00296196