Cellulases and hemicellulases of the anaerobic fungus Piromyces constitute a multiprotein cellulose-binding complex and are encoded by multigene families

More than 80% of the extracellular Avicelase, endoglucanase, xylanase and mannanase activities of the anaerobic fungus Piromyces were associated with a cellulose-binding complex. The complex was composed of at least 10 polypeptides ranging in size from 190 kDa to 50 kDa, and contained numerous endog...

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Bibliographic Details
Published in:FEMS microbiology letters 1995, Vol.125 (1), p.15-22
Main Authors: Ali, B.R.S, Zhou, L, Graves, F.M, Freedman, R.B, Black, G.W, Gilbert, H.J, Hazlewood, G.P
Format: Article
Language:English
Subjects:
Anaerobic fungus
Anaerobiosis
avicelase
beta-mannosidase
binding
Binding Sites
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Carrier Proteins - chemistry
Carrier Proteins - genetics
Carrier Proteins - isolation & purification
Cellulase
Cellulase - genetics
Cellulase - isolation & purification
Cellulase - metabolism
cellulases
cellulose
Cellulose - metabolism
Cellulose‐binding complex
Cloning, Molecular
complementary DNA
DNA libraries
Electrophoresis, Polyacrylamide Gel
endoglucanase
enzyme activity
Escherichia coli
Fundamental and applied biological sciences. Psychology
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - isolation & purification
fungi
Fungi - enzymology
Fungi - genetics
Gene Library
Genes, Fungal
Glycoside Hydrolases - genetics
Glycoside Hydrolases - isolation & purification
Glycoside Hydrolases - metabolism
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Hemicellulase
Microbiology
Mission oriented research
Multigene Family
Mycology
Physiology and metabolism
Piromyces
Piromyces sp
Plasmids
Polysaccharide Hydrolase genes
proteins
Substrate Specificity
xylanase
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Summary:More than 80% of the extracellular Avicelase, endoglucanase, xylanase and mannanase activities of the anaerobic fungus Piromyces were associated with a cellulose-binding complex. The complex was composed of at least 10 polypeptides ranging in size from 190 kDa to 50 kDa, and contained numerous endoglucanases, xylanases and mannanases. Multiple genes encoding each of these activities were isolated from an expressing cDNA library.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1995.tb07329.x