Inhibition of Myogenic Differentiation in Proliferating Myoblasts by Cyclin D1-Dependent Kinase

Although the myogenic regulator MyoD is expressed in proliferating myoblasts, differentiation of these cells is limited to the G$_0$ phase of the cell cycle. Forced expression of cyclin D1, but not cyclins A, B, or E, inhibited the ability of MyoD to transactivate muscle-specific genes and correlate...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1995-02, Vol.267 (5200), p.1022-1024
Main Authors: Skapek, Stephen X., Rhee, James, Spicer, Douglas B., Lassar, Andrew B.
Format: Article
Language:English
Subjects:
Animals
Blasts
Carrier Proteins - biosynthesis
Carrier Proteins - physiology
Cell Cycle
Cell Differentiation
Cell Line
Cell proliferation
Cellular biology
Cultured cells
Cyclin D1
Cyclin-Dependent Kinase Inhibitor p16
Cyclin-Dependent Kinase Inhibitor p21
Cyclin-Dependent Kinases - metabolism
Cyclins
Cyclins - biosynthesis
Cyclins - physiology
Enzyme Activation
Genes
Individualized Instruction
Inhibition
Mice
Muscle cells
Muscle fibers
Muscle, Skeletal - cytology
Muscle, Skeletal - metabolism
Muscular system
Myoblasts
MyoD Protein - antagonists & inhibitors
MyoD Protein - metabolism
Oncogene Proteins - physiology
Phosphorylation
Physiological aspects
Plasmids
Proteins
Transcriptional Activation
Transfection
Vehicles
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Summary:Although the myogenic regulator MyoD is expressed in proliferating myoblasts, differentiation of these cells is limited to the G$_0$ phase of the cell cycle. Forced expression of cyclin D1, but not cyclins A, B, or E, inhibited the ability of MyoD to transactivate muscle-specific genes and correlated with phosphorylation of MyoD. Transfection of myoblasts with cyclin-dependent kinase (Cdk) inhibitors p21 and p16 augmented muscle-specific gene expression in cells maintained in high concentrations of serum, suggesting that an active cyclin-Cdk complex suppresses MyoD function in proliferating cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7863328