A Permanent Glial Precursor Cell Line, Immortalized with the Adenovirus E1A Gene, Undergoes Apoptosis in Restrictive Growth Conditions

We have previously described some differentiation properties of the ERD.1.1 cell line, obtained after transfer and integration of the adenovirus-5 E1A gene. Depending on the growth conditions, these cells differentiate towards the astrocyte or early oligodendrocyte differentiation pathway. However,...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-02, Vol.207 (2), p.630-636
Main Authors: Borde, I., Evrard, C., Galiana, E., Rouget, P.
Format: Article
Language:English
Subjects:
Adenovirus E1A Proteins - genetics
Adenoviruses, Human - genetics
Animals
Apoptosis
Astrocytes - cytology
Cell Differentiation
Cell Division
Cell Line, Transformed
Chromatin - ultrastructure
DNA - analysis
Gene Transfer Techniques
Genes, Viral
Microscopy, Electron
Neuroglia - cytology
Neuroglia - physiology
Neuroglia - ultrastructure
Oligodendroglia - cytology
Virus Integration
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Description
Summary:We have previously described some differentiation properties of the ERD.1.1 cell line, obtained after transfer and integration of the adenovirus-5 E1A gene. Depending on the growth conditions, these cells differentiate towards the astrocyte or early oligodendrocyte differentiation pathway. However, in growth restrictive conditions, we observed dying cells that detached from the monolayer constituted of differentiating cells. This led us to examine the characteristics of the dying cells. The study of the low-molecular-weight DNA and the ultrastructural examination of chromatin showed that these cells were undergoing apoptosis. The results also suggest that the expression of an integrated polyoma middle T gene can partly save the cells from apoptosis.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.1234