An immunocytochemical study of fetal cells at the maternal-placental interface using monoclonal antibodies to keratins, vimentin and desmin

The expression of keratin, vimentin and desmin intermediate filaments by cells in the placenta, amniochorion and placental bed at different stages of pregnancy was studied by use of a panel of monoclonal antibodies. All trophoblast subsets express keratin but not vimentin or desmin intermediate fila...

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Bibliographic Details
Published in:Cell and tissue research 1986-01, Vol.246 (1), p.189-195
Main Authors: KHONG, T. Y, LANE, E. B, ROBERTSON, W. B
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Biological and medical sciences
Cytoskeleton - ultrastructure
Desmin - analysis
Female
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Humans
Immunoenzyme Techniques
Intermediate Filaments - ultrastructure
Keratins - analysis
Maternal-Fetal Exchange
Mother. Fetoplacental unit. Mammary gland. Milk
Placenta - cytology
Pregnancy
Pregnancy. Parturition. Lactation
Trophoblasts - cytology
Vertebrates: reproduction
Vimentin - analysis
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Summary:The expression of keratin, vimentin and desmin intermediate filaments by cells in the placenta, amniochorion and placental bed at different stages of pregnancy was studied by use of a panel of monoclonal antibodies. All trophoblast subsets express keratin but not vimentin or desmin intermediate filaments at all stages of pregnancy. Differentiation of the various forms of trophoblast probably does not involve qualitative alterations to the keratin pattern of embryonic trophoblast. Amniotic epithelium co-expressed keratin and variable amounts of vimentin while a subset of fetal mesenchyme cells of the amniochorion and chorionic villi were immunolabelled by antibodies to keratin, to vimentin and to desmin, suggesting simultaneous triple co-expression of three intermediate filaments. This finding suggests the identification of a cell population that is analogous to parietal endoderm in some eutherian animals.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00219017