Cloning and Expression of Human Lymphotoxin mRNA Derived from a Human T Cell Hybridoma

• Published in: Journal of biochemistry (Tokyo) 1986, Vol.100 (3), p.727-733
• Main Authors: KOBAYASHI, Yoshiro, MIYAMOTO, Daisei, ASADA, Makoto, OBINATA, Masuo, OSAWA, Toshiaki
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; Biotechnology; cDNA; cloning; Cloning, Molecular; DNA - analysis; DNA - genetics; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; gene expression; genes; Genetic engineering; Genetic technics; Humans; hybridoma; Hybridomas; lymphocytes T; lymphotoxin; Lymphotoxin-alpha - biosynthesis; Lymphotoxin-alpha - genetics; man; Methods. Procedures. Technologies; Nucleic Acid Hybridization; nucleotide sequence; Plasmids; RNA, Messenger - analysis; RNA, Messenger - genetics; T-Lymphocytes; transformation
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/oxfordjournals.jbchem.a121765

We cloned human lymphotoxin (LT) cDNA from a cDNA library prepared from phorbol myristate acetate (PMA) and Con A-stimulated human T cell hybridoma (AC5-8) cells. The nucleotide sequence of the cDNA insert in the plasmid, pLT13, was determined and compared with those of peripheral blood mononuclear cell derived LT cDNA and the LT gene. Four stretches, containing 14 nucleotides in total, were different among the three sequences. The differences included one base change from C to A in the coding region. Because this change was expected to result in a change in the amino acid at position 26 from Thr to Asn, we constructed an E. coli expression plasmid (pLT13tac6-8.2) and a mammalian cell expression plasmid (pSV2-LT) in order to confirm that pLT13 contains the coding sequence of human LT. Both plasmids were found to synthesize active LT molecules after transfection into JM1O5 and COS-1 cells, respectively, and their lytic activities were found to be completely neutralized by anti human LT serum. Using an insertion mutant and a deletion mutant, we examined the role of the C terminal domain in the LT activity. The results obtained strongly suggested that the intactness of the C terminal less than 10 residues is required for the LT activity.