Recognition of Human T-Leukemia Virus (HTLV-1) Envelope by Human CD4+ T-Cell Lines From HTLV-1 Seronegative Individuals: Specificity and Clonal Heterogeneity
Because T-helper cells are critical for immune responses in retroviral infections, CD4+ T-cell lines specific for the human T-leukemia virus type 1 (HTLV-1) envelope have been generated from peripheral T lymphocytes of nonimmune donors to study their naive repertoire. Recombinant fragments (RE1, ami...
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Published in: | Blood 1995-03, Vol.85 (6), p.1547-1554 |
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Main Authors: | , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
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Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Because T-helper cells are critical for immune responses in retroviral infections, CD4+ T-cell lines specific for the human T-leukemia virus type 1 (HTLV-1) envelope have been generated from peripheral T lymphocytes of nonimmune donors to study their naive repertoire. Recombinant fragments (RE1, amino acids [aa] 26-200; RE3, aa 165-307; RE5, aa 308-401; and RE6, aa 165-401) of HTLV-1 envelope, whole envelope glycoprotein, and synthetic peptides were used to induce T-cell lines. CD4+ T-cell lines specific for one or more fragments were obtained from seven of eight individuals tested. T-cell lines generated against envelope glycoprotein from five of five donors did not cross-react with the RE fragments and vice versa. The lines specific for RE and env were mapped with overlapping peptides. The lines with single peptide (narrow) specificity contained a variety of clones that used different T-cell receptor Vβ genes. These data (1) suggest that most of the normal individuals carry T-helper precursors specific for epitopes on HTLV-1 envelope; (2) indicate that heterogeneity of HTLV-1 envelope-specific T cells can be detected in the naive repertoire; and (3) define optimal antigenic preparations to be used to assess cellular immunity in HTLV-1–infected individuals. |
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ISSN: | 0006-4971 1528-0020 |
DOI: | 10.1182/blood.V85.6.1547.bloodjournal8561547 |