Recognition of Human T-Leukemia Virus (HTLV-1) Envelope by Human CD4+ T-Cell Lines From HTLV-1 Seronegative Individuals: Specificity and Clonal Heterogeneity

Because T-helper cells are critical for immune responses in retroviral infections, CD4+ T-cell lines specific for the human T-leukemia virus type 1 (HTLV-1) envelope have been generated from peripheral T lymphocytes of nonimmune donors to study their naive repertoire. Recombinant fragments (RE1, ami...

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Published in:Blood 1995-03, Vol.85 (6), p.1547-1554
Main Authors: Manca, Fabrizio, Pira, Giuseppina Li, Fenoglio, Daniela, Valle, Maria Teresa, Kunkl, Annalisa, Ferraris, Anna, Lancia, Flavia, Saverino, Daniele, Mortara, Lorenzo, Balderas, Robert, Arp, Jacqueline, Dekaban, Gregory, Dalgleish, Angus G., Lozzi, Luisa, Theofilopoulos, Argyrios N.
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Language:English
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Summary:Because T-helper cells are critical for immune responses in retroviral infections, CD4+ T-cell lines specific for the human T-leukemia virus type 1 (HTLV-1) envelope have been generated from peripheral T lymphocytes of nonimmune donors to study their naive repertoire. Recombinant fragments (RE1, amino acids [aa] 26-200; RE3, aa 165-307; RE5, aa 308-401; and RE6, aa 165-401) of HTLV-1 envelope, whole envelope glycoprotein, and synthetic peptides were used to induce T-cell lines. CD4+ T-cell lines specific for one or more fragments were obtained from seven of eight individuals tested. T-cell lines generated against envelope glycoprotein from five of five donors did not cross-react with the RE fragments and vice versa. The lines specific for RE and env were mapped with overlapping peptides. The lines with single peptide (narrow) specificity contained a variety of clones that used different T-cell receptor Vβ genes. These data (1) suggest that most of the normal individuals carry T-helper precursors specific for epitopes on HTLV-1 envelope; (2) indicate that heterogeneity of HTLV-1 envelope-specific T cells can be detected in the naive repertoire; and (3) define optimal antigenic preparations to be used to assess cellular immunity in HTLV-1–infected individuals.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V85.6.1547.bloodjournal8561547