Transfection, expression, and DNA sequence of a gene encoding a BoLA-A11 antigen

• Published in: Immunogenetics (New York) 1995-02, Vol.41 (4), p.246-250
• Main Authors: Sawhney, S M, Hasima, N N, Glass, E J, al-Murrani, S W, Nichani, A K, Spooner, R L, Williams, J L, Russell, G C
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Cattle; Gene Expression - genetics; Genes, MHC Class I - genetics; Molecular Sequence Data; Transfection - genetics
• ISSN: 0093-7711; 1432-1211
• DOI: 10.1007/BF00172066

Cattle major histocompatibility complex [(MHC) (BoLA)] class I molecules are heterodimeric glycoproteins which present endogenous antigenic peptides to CD8 super(+) T lymphocytes, initiating a cellular immune response. The MHC-encoded heavy chains are highly polymorphic and, in cattle, have been characterized mainly by using allo-antisera raised by reciprocal calf/dam immunizations. This has enabled the identification of about 50 serological specificities, most of which behave as alleles of a single highly polymorphic class I locus. In order to investigate the expression and function of individual class I locus products, we are correlating BoLA class I gene sequences with the expressed products by the transfection and characterization of genomic class I clones. Shotgun transfection and expression of BoLA class I molecules has been described previously, but the genes involved were not isolated. In this paper we report the isolation, DNA sequencing, transfection, and expression of a genomic clone encoding a BoLA-A11 determinant from an animal expressing A10 and A11 serological specificities.