Sequence and Mapping of Galectin-5, a β-Galactoside-binding Lectin, Found in Rat Erythrocytes (∗)

• Published in: The Journal of biological chemistry 1995-03, Vol.270 (10), p.5032-5038
• Main Authors: Gitt, Michael A., Wiser, Mark F., Leffler, Hakon, Herrmann, Joerg, Xia, Yu-Rong, Massa, Stephen M., Cooper, Douglas N.W., Lusis, Aldons J., Barondes, Samuel H.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Chromatography, Gel; Chromatography, High Pressure Liquid; Cloning, Molecular; DNA Primers; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Erythrocytes - metabolism; Galectins; Lectins - biosynthesis; Lectins - chemistry; Lectins - isolation & purification; Lung - metabolism; Mammals; Molecular Sequence Data; Open Reading Frames; Peptide Fragments - chemistry; Peptide Fragments - isolation & purification; Polymerase Chain Reaction; Protein Conformation; Rats; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - isolation & purification; Restriction Mapping; Reticulocytes - metabolism; Sequence Homology, Amino Acid
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.270.10.5032

A monomeric rat β-galactoside-binding lectin previously purified from extracts of rat lung has been localized to erythrocytes, and the cDNA encoding it has been isolated from a rat reticulocyte cDNA library. The deduced amino acid sequence of the cDNA predicts a protein with a Mr of 16,199, with no evidence of a signal peptide. The deduced sequence is identical to the sequences of seven proteolytic peptides derived from the purified lectin. Peptide analysis by mass spectrometry indicates that the N-terminal methionine is cleaved and that serine 2 is acetylated. The lectin shares all the strictly conserved amino acid residues of other members of the mammalian galectin family and is designated galectin-5 (GenBankTM accession number L36862). Galectin-5 is a weak agglutinin of rat erythrocytes, despite its monomeric structure. The gene encoding galectin-5 (LGALS5) has been mapped in mouse to chromosome 11, ∼50 centimorgans from the centromere and 1.8 ± 1.8 centimorgans from the polymorphic marker D11Mit34n, a region syntenic with human chromosome 17q11.