Transcriptional regulation by p53. Functional interactions among multiple regulatory domains

The tumor suppressor p53 protein possesses activities typical of eukaryotic transcriptional activators; p53 binds to specific DNA sequences and stimulates transcription of the target genes. By a series of deletion and domain-swapping studies, we report here that (i) p53 has two auxiliary domains, wh...

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Bibliographic Details
Published in:The Journal of biological chemistry 1995-03, Vol.270 (12), p.6966-6974
Main Authors: Hsu, Y S, Tang, F M, Liu, W L, Chuang, J Y, Lai, M Y, Lin, Y S
Format: Article
Language:English
Subjects:
Base Sequence
DNA - metabolism
DNA-Binding Proteins
Fungal Proteins - metabolism
Molecular Sequence Data
Saccharomyces cerevisiae Proteins
Transcription Factors
Transcription, Genetic
Tumor Suppressor Protein p53 - physiology
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Summary:The tumor suppressor p53 protein possesses activities typical of eukaryotic transcriptional activators; p53 binds to specific DNA sequences and stimulates transcription of the target genes. By a series of deletion and domain-swapping studies, we report here that (i) p53 has two auxiliary domains, which have little effect on the DNA binding activity of its core domain but are capable of modulating its transactivation activity in a target site-dependent manner; (ii) p53 contains two cell-specific transcriptional inhibitory domains, I1 and I2, which are active in Saos-2 and HeLa cells but not in HepG2 and Hep3B cells; and (iii) I1 inhibits the activity of several structurally different activating regions. These results demonstrate that the apparent transcriptional activity of p53 is determined by collaborations among its regulatory domains, its target sites, and the cellular environment.
ISSN:0021-9258
DOI:10.1074/jbc.270.12.6966