The cDNA cloning and molecular analysis of two self-incompatibility alleles from apple

Complementary DNA clones representing two alleles of the self-incompatibility (S) locus of apple (Malus x domestica Borkh.) have been isolated and characterised. One of the alleles corresponds to a 29 kDa ribonuclease (S-RNase) that was purified from pistil tissue. On northern blots, both cDNAs hybr...

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Bibliographic Details
Published in:Plant molecular biology 1995-02, Vol.27 (3), p.499-511
Main Authors: Broothaerts, W. (Katholieke Univ. Leuven (Belgium). Lab. of Genetics), Janssens, G.A, Proost, P, Broekaert, W.F
Format: Article
Language:English
Subjects:
ADN
Alleles
Amino Acid Sequence
AUTOESTERILIDAD
AUTOSTERILITE
Base Sequence
CLONACION
CLONAGE
CLONING
Cloning, Molecular
Conserved Sequence - genetics
DNA
DNA, Complementary - genetics
DNA, Plant - genetics
Fruit - enzymology
Fruit - genetics
Fruit - physiology
GENE
GENES
Genes, Plant - genetics
GINECEO
GYNECEE
GYNOECIUM
Introns - genetics
MALUS PUMILA
Molecular Sequence Data
RIBONUCLEASAS
RIBONUCLEASE
RIBONUCLEASES
Ribonucleases - genetics
Ribonucleases - isolation & purification
SELF STERILITY
Sequence Alignment
Sequence Homology, Amino Acid
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Summary:Complementary DNA clones representing two alleles of the self-incompatibility (S) locus of apple (Malus x domestica Borkh.) have been isolated and characterised. One of the alleles corresponds to a 29 kDa ribonuclease (S-RNase) that was purified from pistil tissue. On northern blots, both cDNAs hybridized to a transcript that was only present in pistils and not in the other plant tissues analysed. Corresponding genomic sequences, amplified by PCR, were found to contain a single intron of 138 bp and 1100 bp respectively. Comparison of both sequences shows that the cDNAs encode mature proteins containing 65% of identical residues. Eight invariable cysteine residues, conserved regions around two histidines thought to play a role in RNA catalysis, and a number of other distinct residues are conserved between the apple S-RNases and similar proteins in the family Solanaceae. As this is the first report of sequences of S-alleles from a species belonging to a family that is not related with the Solanaceae, the structural features of S-RNases deduced from a comparison of their sequences are discussed.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00019317