Determination of Junction Avidity of Cytolytic T Cell and Target Cell

A direct measurement of the avidity of the junction between a cytotoxic T lymphocyte and its target cell was achieved by using a biophysical approach. A micromanipulation technique was used to determine the force required to separate a cytotoxic T cell (human clone F1, with specificity for HLA-DRw6)...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1986-12, Vol.234 (4782), p.1405-1408
Main Authors: Sung, Kuo-Li P., Sung, Lanping A., Crimmins, Mary, Burakoff, Steven J., Chien, Shu
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Cell Communication
Cell lines
Cell membranes
Critical loading
Cytolysis
Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others)
Cytotoxicity, Immunologic
Dendritic cells
Fundamental and applied biological sciences. Psychology
Fundamental immunology
HLA-DR Antigens - immunology
HLA-DR6 Antigen
Humans
Immunobiology
Immunological reactions in vitro
lymphocytes T
man
Mathematics
membrane junctions
Micromanipulation
Molecular interactions
Phagocytosis
Pipettes
Receptors
T cells
T lymphocytes
T-Lymphocytes, Cytotoxic - cytology
T-Lymphocytes, Cytotoxic - immunology
target cells
Time Factors
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Summary:A direct measurement of the avidity of the junction between a cytotoxic T lymphocyte and its target cell was achieved by using a biophysical approach. A micromanipulation technique was used to determine the force required to separate a cytotoxic T cell (human clone F1, with specificity for HLA-DRw6) from its specific target cell (JY: HLA-A2, -B7, -DR4, w6) prior to delivery of the lethal hit. The force required to separate the F1-JY pair is 1.5 $\times $ 10$^{4}$ dynes per square centimeter. This junction avidity for F1-JY pairs is 6 to 13 times greater than that for F1-F1 and JY-JY pairs; the F1-JY conjugate requires a stronger separating force and is more easily rejoined than the homologous cell pairs. This study provides an estimate of the avidity of cytotoxic T cells for their target cells and insights into the biophysical correlates of the molecular complexes formed in the interaction of cytotoxic T cells and their targets during the cytotoxic process.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3491426