Bordetella pertussis Fimbriae Bind to Human Monocytes via the Minor Fimbrial Subunit FimD

Nonopsonized Bordetella pertussis can bind to and become ingested by human monocytes. Previous studies demonstrated that mutant B. pertussis strains that lack fimbriae express a reduced adherence to monocytes. The present study was undertaken to investigate the involvement of the minor fimbrial subu...

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Bibliographic Details
Published in:The Journal of infectious diseases 1995-04, Vol.171 (4), p.924-929
Main Authors: Hazenbos, Wouter L. W., Geuijen, Cecile A. W., Berg, Bernard M. van den, Mooi, Frits R., Furth, Ralph van
Format: Article
Language:English
Subjects:
Bacteria
Bacterial adhesins
Bacterial Adhesion - physiology
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - metabolism
Bacterial Proteins
Bordetella pertussis
Bordetella pertussis - metabolism
Carrier Proteins - genetics
Cytometry
Fimbriae
Fimbriae Proteins
Fimbriae, Bacterial - metabolism
Flow Cytometry
Genes, Bacterial - genetics
Genetic mutation
Humans
Lymphocytes
Lymphocytes - metabolism
Major Articles
Maltose-Binding Proteins
Monocytes
Monocytes - metabolism
Monocytes - microbiology
Mutation - physiology
Neutrophils
Neutrophils - metabolism
Recombinant Fusion Proteins - biosynthesis
Whooping cough
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Summary:Nonopsonized Bordetella pertussis can bind to and become ingested by human monocytes. Previous studies demonstrated that mutant B. pertussis strains that lack fimbriae express a reduced adherence to monocytes. The present study was undertaken to investigate the involvement of the minor fimbrial subunit FimD in the adherence of B, pertussis to human monocytes using purified fimbriae, FimD, and strains with mutations in fimbrial genes. Flow cytometry demonstrated that purified B. pertussis fimbriae avidly bind to monocytes in a dose-dependent manner but bind less to neutrophils and hardly to lymphocytes; FimD-lacking fimbriae did not bind to monocytes. Purified fimbriae or FimD inhibited the binding of wild-type B, pertussis to monocytes in a dose-dependent manner and similarly inhibited the binding of a mutant defective in the major fimbrial subunits. It did not affect the binding of strains defective in FimD. These results prove that B. pertussis bind to human monocytes via the fimbrial minor subunit FimD.
ISSN:0022-1899
DOI:10.1093/infdis/171.4.924