Relatedness of penicillin-resistant Streptococcus pneumoniae serogroup 9 strains from France and Spain

• Published in: Microbiology (Society for General Microbiology) 1995-03, Vol.141 (3), p.623-627
• Main Authors: Gasc,, Anne-Marie, Geslin, Pierre, Sicard, A. Michel
• Format: Article
• Language: English
• Subjects: Bacteriology; Biological and medical sciences; Deoxyribonucleases, Type II Site-Specific; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Electrophoresis, Gel, Pulsed-Field; France; Fundamental and applied biological sciences. Psychology; Humans; Microbiology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Penicillin Resistance - genetics; Pneumococcal Infections - drug therapy; Pneumococcal Infections - microbiology; Serotyping; Spain; Streptococcus pneumoniae; Streptococcus pneumoniae - classification; Streptococcus pneumoniae - drug effects; Streptococcus pneumoniae - genetics
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/13500872-141-3-623

1 Laboratoire de Microbiologie et Génétique Moléculaire CNRS and Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, France 2 Centre National de Référence des Pneumocoques, Centre Hospitalo-universitaire, 40 avenue de Créteil, 94010 Créteil, France ABSTRACT Summary: Pulsed-field gel electrophoresis of the genomic DNA of penicillin-resistant strains of Streptococcus pneumoniae was carried out. Eleven clinical strains of serogroup 9 from different French towns and Paris hospitals were tested. The restriction enzymes Apal and Smal were used to digest intact chromosomes, and the fragments were resolved by field-inversion gel electrophoresis (FIGE). Five strains were similar using Apal and Smal . Four others were closely related when using Apal , and five others were closely related when using Smal . These results suggest that 10 of these strains are genetically related and have a clonal origin. The profile of the eleventh strain was completely different. Thus, in a given serotype the spreading of penicillin resistance can result from both clonal and independent events. Five strains had similar FIGE profiles to strains first isolated in Spain, suggesting that a resistant strain had spread from Spain to France. 5 Author for corresprespondence: Anne-Marie Gasc. Tel: [phone]. Fax: [phone]. e-mail: [e-mail] Keywords: Streptococcus pneumoniae, fingerprinting, FIGE, penicillin resistance, serotype