Vaccinia virus transfection of hippocampal slice neurons

Here we describe a technique that uses a recombinant vaccinia virus to transfect neurons in rat hippocampal slices. This technique allows the use of molecular biological manipulations on neuronal tissue while maintaining intact synaptic function. This method should be useful in testing specific hypo...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 1995-04, Vol.14 (4), p.685-688
Main Authors: Pettit, D.L, Koothan, Thillai, Liao, Dezhi, Malinow, R
Format: Article
Language:English
Subjects:
Animals
beta-Galactosidase - genetics
Dendrites - metabolism
Electrophysiology
Fluoresceins - metabolism
Fluorescent Dyes
Galactosides - metabolism
Glutathione - metabolism
Hippocampus - cytology
Neurons - metabolism
Neurons - physiology
Rats
Rats, Sprague-Dawley
Recombinant Proteins - analysis
Transfection
vaccinia virus
Vaccinia virus - genetics
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Description
Summary:Here we describe a technique that uses a recombinant vaccinia virus to transfect neurons in rat hippocampal slices. This technique allows the use of molecular biological manipulations on neuronal tissue while maintaining intact synaptic function. This method should be useful in testing specific hypotheses regarding the role of synaptic proteins.
ISSN:0896-6273
1097-4199
DOI:10.1016/0896-6273(95)90212-0