Analysis of basic antimalarial drugs by CZE; Part 2. Validation and application to bioanalysis

• Published in: Journal of pharmaceutical and biomedical analysis 1995, Vol.13 (1), p.21-26
• Main Authors: Taylor, R.B., Reid, R.G.
• Format: Article
• Language: English
• Subjects: antimalarials; Antimalarials - isolation & purification; Calibration; Chloroquine - isolation & purification; Chloroquine - metabolism; Chromatography - methods; CZE; Electrophoresis - methods; Humans; Injections; Proguanil - isolation & purification; Proguanil - metabolism; quantitation; Reproducibility of Results; Sensitivity and Specificity; Vacuum
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/0731-7085(94)00127-N

This report describes some of the quantitative aspects of the CZE separation of proguanil, chloroquine and their respective metabolites, the separations of which, by CE and MEKC, were reported in Part 1. Results obtained on the precision of migration time and peak areas using the alternative injection methods of vacuum and electrokinetic are described and discussed. The increase in concentration sensitivity using electrokinetic injection with an organic injection solvent reported in Part 1 is confirmed and the resultant limits of detection in urine reported. An assay method for these compounds in urine is described which incorporates a pretreatment stage of solid phase extraction and the main analytical parameters used in the validation of such an assay are reported. The limitation of the sample pretreatment used when applied to matrices of plasma and saliva are reported and discussed in the context of the electrokinetic injection method used.