Cloning, sequencing and localization to chromosome 11 of a cDNA encoding a human opioid-binding cell adhesion molecule (OBCAM)

Oligodeoxyribonucleotide (oligo) primers derived from rat opioid-binding cell adhesion molecule (OBCAM)-encoding cDNA sequence were used to amplify a 403-bp fragment from a human brain cDNA library using the polymerase chain reaction (PCR). The fragment was cloned, sequenced and used as a hybridizat...

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Bibliographic Details
Published in:Gene 1995-04, Vol.155 (2), p.213-217
Main Authors: Shark, Katherine B., Lee, Nancy M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
bovine
Carrier Proteins - genetics
Cell Adhesion Molecules - genetics
Chromosome Mapping
Chromosomes, Human, Pair 11 - genetics
Cloning, Molecular
DNA, Complementary - genetics
GPI-Linked Proteins
Humans
Molecular Sequence Data
opioid receptor
rat
Recombinant DNA
Sequence Alignment
λ, library screening
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Summary:Oligodeoxyribonucleotide (oligo) primers derived from rat opioid-binding cell adhesion molecule (OBCAM)-encoding cDNA sequence were used to amplify a 403-bp fragment from a human brain cDNA library using the polymerase chain reaction (PCR). The fragment was cloned, sequenced and used as a hybridization probe to screen the library. λ plaque clones were isolated which contained a 1.5-kb cDNA fragment, including a complete open reading frame (ORF) of 1038 bp. Sequence analysis of the ORF revealed 93% identity to the rat OBCAM cDNA at the nucleotide level, and the deduced amino-acid sequences shared 98% identity. Percentages of identity between human and bovine OBCAM ORFs were within 2% of these values. OBCAM was mapped to human chromosome 11 by hybridizing the probe with a somatic cell hybrid panel
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(94)00830-L