Specific monoclonal antibodies neutralize the action of PACAP 1–27 or PACAP 1–38 on intestinal muscle strips in vitro

The gut-brain neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) is a novel highly conserved member of the secretin-glucagon-VIP peptide family comprising 38 or 27 amino acid residues. In this study, we investigate the actions of PACAP 1–27 or PACAP 1–38 on jejunal and caecal mu...

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Bibliographic Details
Published in:Regulatory peptides 1995-01, Vol.55 (1), p.57-66
Main Authors: Schwarzhoff, Rainer, Schwörer, Harald, Fornefeld, Heike, Morys-Wortmann, Corinna, Katsoulis, Stavros, Creutzfeldt, Werner, Fölsch, Ulrich R., Schmidt, Wolfgang E.
Format: Article
Language:English
Subjects:
Animals
Antagonist
Antibodies, Monoclonal - immunology
Biological and medical sciences
Cecum - physiology
Dose-Response Relationship, Drug
Fundamental and applied biological sciences. Psychology
Gastrointestinal motility
Gastrointestinal Motility - drug effects
Guanethidine - pharmacology
Guinea Pigs
Hybridoma
In vitro neutralization
In Vitro Techniques
Intestine. Mesentery
Jejunum - physiology
Muscle Relaxation - drug effects
Muscle, Smooth - drug effects
Muscle, Smooth - physiology
Neuropeptides - immunology
Neuropeptides - pharmacology
Neuropeptides - physiology
Pituitary Adenylate Cyclase-Activating Polypeptide
Receptor
Scopolamine - pharmacology
Swine
Vertebrates: digestive system
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Summary:The gut-brain neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) is a novel highly conserved member of the secretin-glucagon-VIP peptide family comprising 38 or 27 amino acid residues. In this study, we investigate the actions of PACAP 1–27 or PACAP 1–38 on jejunal and caecal muscle strips from pig or guinea pig and demonstrate the neutralizing effect of two PACAP-specific monoclonal antibodies of the IgG 1 subtype, RSP27II and RSP38. These antibodies were used to set up assay systems specific for PACAP 1–27 or PACAP 1–38. Monoclonal antibody RSP27II recognizes exclusively PACAP 1–27, whereas RSP38 binds only PACAP 1–38. PACAP 1–27 and PACAP 1–38 relax taenia caeci dose-dependently in the presence of guanethidine and scopolamine. Both peptides inhibit the spontaneous contractions of porcine jejunal muscle strips equipotently. Monoclonal antibodies RSP27II and RSP38 specifically neutralize the actions of either exogenously applied or endogenously released PACAP. Thus, they represent processing-specific tools to examine the physiological role of both molecular forms of PACAP in the gastrointestinal tract.
ISSN:0167-0115
1873-1686
DOI:10.1016/0167-0115(94)00092-C