A Flow System for the Study of Shear Forces Upon Cultured Endothelial Cells

A parallel plate chamber in a flow system has been designed to study the effects of fluid shear stresses on cells. The system was applied to the study of cultured endothelial cells grown on cover slips which were accommodated in recessed wells in the base plate. Dye injection studies in the chamber...

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Bibliographic Details
Published in:Journal of biomechanical engineering 1986-11, Vol.108 (4), p.338-341
Main Authors: Koslow, A. R, Stromberg, R. R, Friedman, L. I, Lutz, R. J, Hilbert, S. L, Schuster, P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomechanical Phenomena
Biomechanics. Biorheology
Biomedical Engineering
Cells, Cultured
Endothelium - cytology
Endothelium - physiology
Fundamental and applied biological sciences. Psychology
Hemodynamics
Humans
Models, Cardiovascular
Space life sciences
Stress, Mechanical
Tissues, organs and organisms biophysics
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Summary:A parallel plate chamber in a flow system has been designed to study the effects of fluid shear stresses on cells. The system was applied to the study of cultured endothelial cells grown on cover slips which were accommodated in recessed wells in the base plate. Dye injection studies in the chamber indicated laminar flow over the cells. Shear rates measured over the cover slips by an electrochemical technique were found to be linear with flow rate. Laser doppler anemometry showed parabolic profiles between the plates. Endothelial cells subjected to flow showed a correlation between the time required for orientation and the magnitude of the shear stress.
ISSN:0148-0731
1528-8951
DOI:10.1115/1.3138625