Growth of Measles Virus in Epithelial and Lymphoid Tissues of Cynomolgus Monkeys

In the present study, characteristics of the growth of wild measles virus in the epithelial and lymphoid cells of cynomolgus monkeys (Macaca fascicularis ) were compared by the immunofluorescence (IF) technique and electron microscopy. Pooled throat washings obtained from several patients with measl...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1986, Vol.30(10), pp.1067-1073
Main Authors: Sakaguchi, Masahiro, Yoshikawa, Yasuhiro, Yamanouchi, Kazuya, Sata, Tetsutaro, Nagashima, Kazuo, Takeda, Kazumasa
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Capsid
Epithelium - microbiology
Fundamental and applied biological sciences. Psychology
Lymph Nodes - microbiology
Lymph Nodes - pathology
Lymphocytes - microbiology
Macaca fascicularis
measles virus
Measles virus - growth & development
Measles virus - ultrastructure
Microbiology
Microscopy, Electron
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Trachea - microbiology
Viral Core Proteins
Virology
Virus Replication
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Summary:In the present study, characteristics of the growth of wild measles virus in the epithelial and lymphoid cells of cynomolgus monkeys (Macaca fascicularis ) were compared by the immunofluorescence (IF) technique and electron microscopy. Pooled throat washings obtained from several patients with measles a few days after the onset of rash were used as the source of wild measles virus as described previously. Two cynomolgus monkeys free of measles-virus neutralizing antibody were subcutaneously inoculated with the throat washings. Seven days later, after euthansia, tissues were removed, and IF procedures performed. For the IF technique, the tissues were quickly frozen in n-hexane in a dry-ice-acetone bath. Thin sections made with a cryostat were fixed in acetone, and mixed with monoclonal antibodies against the Edmonston strain of measles virus including three clones against hemagglutinin (H) protein, one clone against nucleocapsid-associated phosphorylated (P) protein, one clone against nucleocapsid (NP) protein, two clones against fusion (F) proteins and two clones against membrane (M) protein (8), and then with goat anti-mouse IgG conjugated with fluorescein.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1986.tb03036.x