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Growth of Measles Virus in Epithelial and Lymphoid Tissues of Cynomolgus Monkeys
In the present study, characteristics of the growth of wild measles virus in the epithelial and lymphoid cells of cynomolgus monkeys (Macaca fascicularis ) were compared by the immunofluorescence (IF) technique and electron microscopy. Pooled throat washings obtained from several patients with measl...
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| Published in: | MICROBIOLOGY and IMMUNOLOGY 1986, Vol.30(10), pp.1067-1073 |
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| Main Authors: | , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c7536-8d910da5eb6227da8d38444681f375e1d92ae044c6e96d5f3ecf821aadb3a3473 |
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| cites | cdi_FETCH-LOGICAL-c7536-8d910da5eb6227da8d38444681f375e1d92ae044c6e96d5f3ecf821aadb3a3473 |
| container_end_page | 1073 |
| container_issue | 10 |
| container_start_page | 1067 |
| container_title | MICROBIOLOGY and IMMUNOLOGY |
| container_volume | 30 |
| creator | Sakaguchi, Masahiro Yoshikawa, Yasuhiro Yamanouchi, Kazuya Sata, Tetsutaro Nagashima, Kazuo Takeda, Kazumasa |
| description | In the present study, characteristics of the growth of wild measles virus in the epithelial and lymphoid cells of cynomolgus monkeys (Macaca fascicularis ) were compared by the immunofluorescence (IF) technique and electron microscopy. Pooled throat washings obtained from several patients with measles a few days after the onset of rash were used as the source of wild measles virus as described previously. Two cynomolgus monkeys free of measles-virus neutralizing antibody were subcutaneously inoculated with the throat washings. Seven days later, after euthansia, tissues were removed, and IF procedures performed. For the IF technique, the tissues were quickly frozen in n-hexane in a dry-ice-acetone bath. Thin sections made with a cryostat were fixed in acetone, and mixed with monoclonal antibodies against the Edmonston strain of measles virus including three clones against hemagglutinin (H) protein, one clone against nucleocapsid-associated phosphorylated (P) protein, one clone against nucleocapsid (NP) protein, two clones against fusion (F) proteins and two clones against membrane (M) protein (8), and then with goat anti-mouse IgG conjugated with fluorescein. |
| doi_str_mv | 10.1111/j.1348-0421.1986.tb03036.x |
| format | article |
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Thin sections made with a cryostat were fixed in acetone, and mixed with monoclonal antibodies against the Edmonston strain of measles virus including three clones against hemagglutinin (H) protein, one clone against nucleocapsid-associated phosphorylated (P) protein, one clone against nucleocapsid (NP) protein, two clones against fusion (F) proteins and two clones against membrane (M) protein (8), and then with goat anti-mouse IgG conjugated with fluorescein.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1986.tb03036.x</identifier><identifier>PMID: 3796316</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Animals ; Biological and medical sciences ; Capsid ; Epithelium - microbiology ; Fundamental and applied biological sciences. Psychology ; Lymph Nodes - microbiology ; Lymph Nodes - pathology ; Lymphocytes - microbiology ; Macaca fascicularis ; measles virus ; Measles virus - growth & development ; Measles virus - ultrastructure ; Microbiology ; Microscopy, Electron ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Trachea - microbiology ; Viral Core Proteins ; Virology ; Virus Replication</subject><ispartof>MICROBIOLOGY and IMMUNOLOGY, 1986, Vol.30(10), pp.1067-1073</ispartof><rights>Center for Academic Publications Japan</rights><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c7536-8d910da5eb6227da8d38444681f375e1d92ae044c6e96d5f3ecf821aadb3a3473</citedby><cites>FETCH-LOGICAL-c7536-8d910da5eb6227da8d38444681f375e1d92ae044c6e96d5f3ecf821aadb3a3473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7968410$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3796316$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakaguchi, Masahiro</creatorcontrib><creatorcontrib>Yoshikawa, Yasuhiro</creatorcontrib><creatorcontrib>Yamanouchi, Kazuya</creatorcontrib><creatorcontrib>Sata, Tetsutaro</creatorcontrib><creatorcontrib>Nagashima, Kazuo</creatorcontrib><creatorcontrib>Takeda, Kazumasa</creatorcontrib><title>Growth of Measles Virus in Epithelial and Lymphoid Tissues of Cynomolgus Monkeys</title><title>MICROBIOLOGY and IMMUNOLOGY</title><addtitle>Microbiology and Immunology</addtitle><description>In the present study, characteristics of the growth of wild measles virus in the epithelial and lymphoid cells of cynomolgus monkeys (Macaca fascicularis ) were compared by the immunofluorescence (IF) technique and electron microscopy. Pooled throat washings obtained from several patients with measles a few days after the onset of rash were used as the source of wild measles virus as described previously. Two cynomolgus monkeys free of measles-virus neutralizing antibody were subcutaneously inoculated with the throat washings. Seven days later, after euthansia, tissues were removed, and IF procedures performed. For the IF technique, the tissues were quickly frozen in n-hexane in a dry-ice-acetone bath. Thin sections made with a cryostat were fixed in acetone, and mixed with monoclonal antibodies against the Edmonston strain of measles virus including three clones against hemagglutinin (H) protein, one clone against nucleocapsid-associated phosphorylated (P) protein, one clone against nucleocapsid (NP) protein, two clones against fusion (F) proteins and two clones against membrane (M) protein (8), and then with goat anti-mouse IgG conjugated with fluorescein.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Capsid</subject><subject>Epithelium - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lymph Nodes - microbiology</subject><subject>Lymph Nodes - pathology</subject><subject>Lymphocytes - microbiology</subject><subject>Macaca fascicularis</subject><subject>measles virus</subject><subject>Measles virus - growth & development</subject><subject>Measles virus - ultrastructure</subject><subject>Microbiology</subject><subject>Microscopy, Electron</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Trachea - microbiology</subject><subject>Viral Core Proteins</subject><subject>Virology</subject><subject>Virus Replication</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNqVkV1v0zAUhi0EGmXwE5AihLhL8FdshyumanSTUhiowKXlxs7qzkmKnWrNv8chVcQdwhe2pfOcx0evAXiDYIbier_PEKEihRSjDBWCZf0WEkhYdnoCFnPpKVhAIvI0ZxA-By9C2EOIORb0AlwQXjCC2ALcrXz32O-Srk7WRgVnQvLD-mNIbJtcH2y_M84ql6hWJ-XQHHad1cnGhnCMYOxZDm3XdO4-Nqy79sEM4SV4VisXzKvzeQm-f7reLG_S8svqdnlVphXPCUuFLhDUKjdbhjHXSmgiKKVMoJrw3CBdYGUgpRUzBdN5TUxVC4yU0luiCOXkErybvAff_YrT9LKxoTLOqdZ0xyA5x7ggef5PEFGGC0pQBD9MYOW7ELyp5cHbRvlBIijH3OVejuHKMVw55i7PuctTbH59fuW4bYyeW89Bx_rbc12FSrnaq7ayYcYiJSiCEfs4YY_WmeE_BpDr2_Wfa1TcTIp96NW9mR3K97ZyRjbxLy0qOJcEjuJpR5DxGal2ykvTRlU6qWzozekv04NkPH6T_Pl5JTfiW35XLkv5lfwGA8bHvQ</recordid><startdate>19860101</startdate><enddate>19860101</enddate><creator>Sakaguchi, Masahiro</creator><creator>Yoshikawa, Yasuhiro</creator><creator>Yamanouchi, Kazuya</creator><creator>Sata, Tetsutaro</creator><creator>Nagashima, Kazuo</creator><creator>Takeda, Kazumasa</creator><general>Blackwell Publishing Ltd</general><general>Center For Academic Publications Japan</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19860101</creationdate><title>Growth of Measles Virus in Epithelial and Lymphoid Tissues of Cynomolgus Monkeys</title><author>Sakaguchi, Masahiro ; Yoshikawa, Yasuhiro ; Yamanouchi, Kazuya ; Sata, Tetsutaro ; Nagashima, Kazuo ; Takeda, Kazumasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c7536-8d910da5eb6227da8d38444681f375e1d92ae044c6e96d5f3ecf821aadb3a3473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Capsid</topic><topic>Epithelium - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lymph Nodes - microbiology</topic><topic>Lymph Nodes - pathology</topic><topic>Lymphocytes - microbiology</topic><topic>Macaca fascicularis</topic><topic>measles virus</topic><topic>Measles virus - growth & development</topic><topic>Measles virus - ultrastructure</topic><topic>Microbiology</topic><topic>Microscopy, Electron</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Trachea - microbiology</topic><topic>Viral Core Proteins</topic><topic>Virology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakaguchi, Masahiro</creatorcontrib><creatorcontrib>Yoshikawa, Yasuhiro</creatorcontrib><creatorcontrib>Yamanouchi, Kazuya</creatorcontrib><creatorcontrib>Sata, Tetsutaro</creatorcontrib><creatorcontrib>Nagashima, Kazuo</creatorcontrib><creatorcontrib>Takeda, Kazumasa</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakaguchi, Masahiro</au><au>Yoshikawa, Yasuhiro</au><au>Yamanouchi, Kazuya</au><au>Sata, Tetsutaro</au><au>Nagashima, Kazuo</au><au>Takeda, Kazumasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth of Measles Virus in Epithelial and Lymphoid Tissues of Cynomolgus Monkeys</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>1986-01-01</date><risdate>1986</risdate><volume>30</volume><issue>10</issue><spage>1067</spage><epage>1073</epage><pages>1067-1073</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>In the present study, characteristics of the growth of wild measles virus in the epithelial and lymphoid cells of cynomolgus monkeys (Macaca fascicularis ) were compared by the immunofluorescence (IF) technique and electron microscopy. Pooled throat washings obtained from several patients with measles a few days after the onset of rash were used as the source of wild measles virus as described previously. Two cynomolgus monkeys free of measles-virus neutralizing antibody were subcutaneously inoculated with the throat washings. Seven days later, after euthansia, tissues were removed, and IF procedures performed. For the IF technique, the tissues were quickly frozen in n-hexane in a dry-ice-acetone bath. Thin sections made with a cryostat were fixed in acetone, and mixed with monoclonal antibodies against the Edmonston strain of measles virus including three clones against hemagglutinin (H) protein, one clone against nucleocapsid-associated phosphorylated (P) protein, one clone against nucleocapsid (NP) protein, two clones against fusion (F) proteins and two clones against membrane (M) protein (8), and then with goat anti-mouse IgG conjugated with fluorescein.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><pmid>3796316</pmid><doi>10.1111/j.1348-0421.1986.tb03036.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0385-5600 |
| ispartof | MICROBIOLOGY and IMMUNOLOGY, 1986, Vol.30(10), pp.1067-1073 |
| issn | 0385-5600 1348-0421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77229355 |
| source | Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Capsid Epithelium - microbiology Fundamental and applied biological sciences. Psychology Lymph Nodes - microbiology Lymph Nodes - pathology Lymphocytes - microbiology Macaca fascicularis measles virus Measles virus - growth & development Measles virus - ultrastructure Microbiology Microscopy, Electron Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Trachea - microbiology Viral Core Proteins Virology Virus Replication |
| title | Growth of Measles Virus in Epithelial and Lymphoid Tissues of Cynomolgus Monkeys |
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