Endothelin-Mediated Positive Inotropic Effect Induced by Reactive Oxygen Species in Isolated Cardiac Muscle

• Published in: Circulation research 1995-05, Vol.76 (5), p.878-884
• Main Authors: De Keulenaer, Gilles W, Andries, Luc J, Sys, Stanislas U, Brutsaert, Dirk L
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cats; Cell Survival - drug effects; Endothelins - biosynthesis; Endothelins - pharmacology; Endothelium - drug effects; Endothelium - metabolism; Fundamental and applied biological sciences. Psychology; Heart; Heart Ventricles - metabolism; In Vitro Techniques; Microscopy, Confocal; Myocardial Contraction - drug effects; Reactive Oxygen Species - pharmacology; Vertebrates: cardiovascular system
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/01.res.76.5.878

Cardiac endothelium, both coronary and endocardial, produces a number of inotropic molecules. Changes in cardiac endothelial function by substances in the superfusing blood may thus participate in the control of muscle-pump performance of the heart. Reactive oxygen species (ROS) have been implicated in normal and pathological vascular physiology by influencing vascular endothelial function. Therefore, we examined the influence of ROS on endocardial endothelial modulation of myocardial performance. Right ventricular cat papillary muscles were briefly (15 s) exposed to electrolysis-generated ROS. Peak total isometric twitch tension and peak rate of tension development increased by 7.8 plus minus 0.7% (P less than .05) and 9.7 plus minus 1.5% (P less than .05), respectively (n equals 12). Isometric twitch duration was slightly increased (time from stimulus to half isometric relaxation, plus 2.7 plus minus 0.6%; P less than .05). ROS scavengers such as ascorbic acid (n equals 6), superoxide dismutase and catalase (n equals 8), or catalase alone (n equals 6), but not superoxide dismutase alone (n equals 6), blocked the inotropic effect. Interestingly, the positive inotropic effect was completely blocked by selectively damaging endocardial endothelium (Triton X-100, 0.5%, 1-s immersion, n equals 7) before ROS generation and by preincubating the muscles with the endothelin-A receptor antagonist BQ 123 (n equals 11). Preincubation with N-nitro-L-arginine methyl ester and indomethacin (n equals 5) or with atenolol (n equals 6) did not influence the inotropic effect. Confocal scanning laser microscopic observations of muscles stained with viability tracers (n equals 9) revealed that significantly more but not all endocardial endothelial cells were damaged in electrolysis-treated muscles than in control muscles (42 plus minus 5% versus 14 plus minus 4%, P less than .05). Accordingly, brief exposure of isolated cardiac muscle to electrolysis-generated ROS damaged the endocardial surface in part and increased contractile performance by stimulating endothelin release from endocardial endothelium. Hence, ROS-induced endothelin release from endocardial endothelium may be involved in normal and/or disturbed regulation of cardiac function.(Circ Res. 1995;76:878-884.)