Validation of an assay to measure adrenocorticotropin in plasma and from chicken leukocytes

A RIA for mammalian adrenocorticotropic hormone (ACTH) was modified and validated to measure chicken ACTH. The assay was capable of detecting an increase in chicken plasma ACTH following treatments known to increase plasma ACTH. Both splenic and peripheral blood leukocytes stimulated with corticotro...

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Bibliographic Details
Published in:Poultry science 1995-02, Vol.74 (2), p.337-342
Main Authors: Hendricks, G.L. III (Pennsylvania State University, University Park, PA.), Mashaly, M.M, Siegel, H.S
Format: Article
Language:English
Subjects:
Adrenocorticotropic Hormone - analysis
Adrenocorticotropic Hormone - drug effects
Adrenocorticotropic Hormone - metabolism
Animals
BAZO
Chickens
CORTICOTROPHINE
Corticotropin-Releasing Hormone - pharmacology
CORTICOTROPINA
EXPERIMENTACION EN LABORATORIO
EXPERIMENTATION EN LABORATOIRE
Hot Temperature - adverse effects
INFECCION
INFECTION
LEUCOCITOS
LEUCOCYTE
Leukocytes - drug effects
Leukocytes - metabolism
Male
METHODE
METODOS
POLLO
POULET
Radioimmunoassay - veterinary
RATE
Sensitivity and Specificity
Stress, Physiological - veterinary
TECHNIQUE RADIOIMMUNOLOGIQUE
TECNICAS RADIOINMUNOLOGICAS
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Description
Summary:A RIA for mammalian adrenocorticotropic hormone (ACTH) was modified and validated to measure chicken ACTH. The assay was capable of detecting an increase in chicken plasma ACTH following treatments known to increase plasma ACTH. Both splenic and peripheral blood leukocytes stimulated with corticotropin-releasing factor (CRF) showed a significant increase in ACTH production compared with unstimulated leukocytes. This finding supports the conclusion that the substance produced by leukocytes previously shown in our laboratory to stimulate adrenal cells to secrete corticosterone is immunoreactive ACTH
ISSN:0032-5791
1525-3171
DOI:10.3382/ps.0740337