Monitoring 5HT Metabolism in the Brain of the Freely Moving Rat

The techniques of repeated CSF withdrawal and intracerebral dialysis are described. They were used to monitor 5HT metabolism in the brains of freely moving rats. Evidence indicates that results obtained proportionately reflect changes of 5HT metabolism in whole brain (CSF method) and in the striatum...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1986-12, Vol.473 (1), p.321-336
Main Authors: HUTSON, P. H., SARNA, G. S., SAHAKIAN, B. J., DOURISH, C. T., CURZON, G.
Format: Article
Language:English
Subjects:
Animals
Brain - drug effects
Brain - metabolism
Dialysis
Eating
Hydroxyindoleacetic Acid - cerebrospinal fluid
Hypothalamus - metabolism
Kinetics
Male
Probenecid - pharmacology
Rats
Rats, Inbred Strains
Serotonin - metabolism
Social Behavior
Tryptophan - pharmacology
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Summary:The techniques of repeated CSF withdrawal and intracerebral dialysis are described. They were used to monitor 5HT metabolism in the brains of freely moving rats. Evidence indicates that results obtained proportionately reflect changes of 5HT metabolism in whole brain (CSF method) and in the striatum (intracerebral dialysis). The potential value of the methods is illustrated by two behavioral studies. The first suggested that 5HT turnover values (determined two weeks prior to behavioral testing by the CSF method) were predictive for neck + body biting in a social interaction test. The second study showed a consistent pattern of change of hypothalamic dialysate 5HIAA concentration associated with feeding in rats on a restricted food intake schedule.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1986.tb23626.x