Glycoprotein Complexes Interacting with Cellulose in the "Cell Print" Zones of the Dictyostelium discoideum Extracellular Matrix

Cellulose is one of the commonest structural biopolymers. How cellulose is organized in extracellular matrices is a mystery. Here we investigate a model system, the extracellular matrix (ECM) of Dictyostelium discoideum which is composed of proteins and cellulose. A group of glycoproteins, the sheat...

Full description

Saved in:
Bibliographic Details
Published in:Developmental biology 1995-04, Vol.168 (2), p.332-341
Main Authors: Zhou-Chou, Ti, Wilkins, Marc R., Vardy, Phil H., Gooley, Andrew A., Williams, Keith L.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cellulose - chemistry
Dictyostelium - chemistry
Dictyostelium discoideum
Extracellular Matrix - chemistry
Glycoproteins - chemistry
Molecular Sequence Data
Molecular Weight
Sequence Alignment
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cellulose is one of the commonest structural biopolymers. How cellulose is organized in extracellular matrices is a mystery. Here we investigate a model system, the extracellular matrix (ECM) of Dictyostelium discoideum which is composed of proteins and cellulose. A group of glycoproteins, the sheathins, which colocalize with cellulose in the ECM of D. discoideum are characterized. Sheathins are dimeric or trimeric forms of molecular mass 53-68 kDa, where the monomers are 12-35 kDa. The sheathin subunits are similar but not identical proteins. The sheathin family comprises sheathin 68, (68-kDa trimer); sheathin 62, (62-kDa dimer); sheathin 55, (55-kDa dimer), and sheathin 53, (53-kDa dimer). The subunits which assemble into the four sheathins represent at least three gene products: ShC, ShD, and ShE which are linked by disulphide bonds, Protein sequence analysis shows two of the sheathin genes encode products ShC and ShD with very similar amino terminal sequences. This group of D. discoideum ECM glycoproteins has homology with two other much larger ECM proteins of D. discoideum, ST430 and ST310, which are located in a more dispersed fashion in the ECM. Sheathins are tightly but non-covalently associated with the ECM, and this association requires strong denaturing conditions for disruption, e.g., SDS or 8 M urea. Sheathins form a component of the "cell prints" which are believed to have a role in cell-ECM interactions and slug cell migration.
ISSN:0012-1606
1095-564X
DOI:10.1006/dbio.1995.1084