Phosphorylation of human plasminogen activators and plasminogen

Plasminogen (PG), urokinase-type plasminogen activator (u-PA) and tissue-type PA (t-PA) are the main molecules involved in fibrinolysis and in many other physiological and pathological processes. In the present study we report that human t-PA, purified from human melanoma cells, and PG, purified fro...

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Bibliographic Details
Published in:FEBS letters 1995-04, Vol.363 (1), p.170-174
Main Authors: Barlati, Sergio, De Petro, Giuseppina, Bona, Chiara, Paracini, Francesco, Tonelli, Mariella
Format: Article
Language:English
Subjects:
5-bromo-4-chloro-3-indolyl-phosphate
alkaline phosphatase
Antibodies, Monoclonal
BCIP
Chromatography, High Pressure Liquid
high pressure liquid chromatography
HPLC
Human plasminogen
Human tissue-type plasminogen activator
Humans
Immunoblotting
Melanoma
MoAb
monoclonal antibodies
NBT
nitroblue tetrazolium
Phosphorylation
Phosphoserine - analysis
Phosphoserine - metabolism
Phosphotyrosine
plasminogen
Plasminogen - metabolism
Plasminogen Activators - metabolism
SDS-PAGE
sodium dodecyl sulphate-polyacrilamide gel electrophoresis
t-PA/u-PA
Tissue Plasminogen Activator - metabolism
tissue-type/urokinase-type plasminogen activator
Tumor Cells, Cultured
Tyrosine - analogs & derivatives
Tyrosine - analysis
Tyrosine - metabolism
Urokinase-type plasminogen activator
Urokinase-Type Plasminogen Activator - metabolism
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Summary:Plasminogen (PG), urokinase-type plasminogen activator (u-PA) and tissue-type PA (t-PA) are the main molecules involved in fibrinolysis and in many other physiological and pathological processes. In the present study we report that human t-PA, purified from human melanoma cells, and PG, purified from human plasma, both contain P-Tyr residues, as revealed by immunoblotting analyses with monoclonal anti-P-Tyr antibodies. In addition HPLC amino acid analysis of acid-hydrolyzed t-PA, PG and u-PA, shows that: (i)P-Ser and P-Tyr residues are present in t-PA; (ii)P-Thr and P-Tyr are present in PG; (iii) P-Ser, P-Thr and P-Tyr are present in u-PA. The utilization of monoclonal anti-P-Ser and anti-P-Thr antibodies in immunoblotting experiments has confirmed these data which indicate that phosphorylation is a common feature of PAs and of PG.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)00312-W