Accumulation of genetic abnormalities during neoplastic progression in Barrett's esophagus

Sex chromosome status, ploidy, and proliferation rate were evaluated in archival material of 73 Barrett's esophagus patients (48 males and 25 females). Diagnosis in esophageal mucosa samples ranged from intestinal metaplasia with no dysplasia to invasive esophageal adenocarcinoma; also, four ly...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1995-05, Vol.55 (9), p.1971-1976
Main Authors: KRISHNADATH, K. K, TILANUS, H. W, VAN BLANKENSTEIN, M, HOP, W. C. J, TEIJGEMAN, R, MULDER, A. H, BOSMAN, F. T, VAN DEKKEN, H
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Barrett Esophagus - genetics
Barrett Esophagus - pathology
Biological and medical sciences
Cell Division - physiology
Chromosome Aberrations
Chromosomes, Human, Pair 1
Disease Progression
DNA Probes
DNA, Neoplasm - analysis
DNA, Neoplasm - genetics
Esophageal Neoplasms - genetics
Esophageal Neoplasms - pathology
Esophagus
Female
Flow Cytometry
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Immunohistochemistry
In Situ Hybridization
Ki-67 Antigen
Male
Medical sciences
Neoplasm Proteins - analysis
Neoplasm Proteins - metabolism
Nuclear Proteins - analysis
Nuclear Proteins - metabolism
Ploidies
Sex Chromosome Aberrations
Tumors
X Chromosome
Y Chromosome
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Summary:Sex chromosome status, ploidy, and proliferation rate were evaluated in archival material of 73 Barrett's esophagus patients (48 males and 25 females). Diagnosis in esophageal mucosa samples ranged from intestinal metaplasia with no dysplasia to invasive esophageal adenocarcinoma; also, four lymph node metastases were studied. Chromosomal and ploidy aberrations were determined by in situ hybridization with repetitive DNA probes specific for chromosomes Y, X, and 1. Proliferation index (Ki-67 protein expression) was assessed by immunohistochemistry. Proliferation rate was elevated in all stages of dysplasia and in the adenocarcinomas. Aneuploidy (hyperdiploidy) and loss of the Y chromosome correlated with the advancing stages toward neoplasia (P < 0.001) and reached high prevalences (70-100%) in high-grade dysplasia and adenocarcinoma. Abnormalities of the X chromosome were not seen. These data suggest that in Barrett's esophagus, genetic perturbations may be generated in relation to a high proliferation rate.
ISSN:0008-5472
1538-7445