Further characterization of the 5'-flanking region of the rat lactase-phlorizin hydrolase gene

The lactase-phlorizin hydrolase gene is widely used as a marker of intestinal differentiation. Recent evidence demonstrating that transcription plays a major role in the regulation of this gene suggests that study of the 5'-flanking region will allow an understanding of how the expression of th...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-04, Vol.209 (3), p.989-995
Main Authors: Verhave, M, Krasinski, S D, Maas, S M, Smiers, F J, Mishra, K, Breeuwsma, N G, Naim, H Y, Grand, R J
Format: Article
Language:English
Subjects:
Animals
Base Sequence
DNA Primers
Genes, Regulator
Genomic Library
Jejunum - enzymology
Lactase-Phlorizin Hydrolase - biosynthesis
Lactase-Phlorizin Hydrolase - genetics
Molecular Sequence Data
Promoter Regions, Genetic
Rats - genetics
Restriction Mapping
RNA - genetics
TATA Box
Transcription, Genetic
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Summary:The lactase-phlorizin hydrolase gene is widely used as a marker of intestinal differentiation. Recent evidence demonstrating that transcription plays a major role in the regulation of this gene suggests that study of the 5'-flanking region will allow an understanding of how the expression of this gene is controlled. However, sequence, restriction, and primer extension analysis of a rat genomic clone has revealed that previously published data are incomplete. In the present study, we used a directed sequencing strategy to carefully analyze this region. Our expanded analysis of the 5'-flanking region of the lactase-phlorizin hydrolase gene should facilitate future studies of its structure and function.
ISSN:0006-291X