T-cell clonality in synovial fluid from rheumatoid joints before and after culture in interleukin-2

T-cell receptor (TCR) gamma gene rearrangements which have been amplified in polymerase chain reactions (PCRs) and analysed by high resolution polyacrylamide gel electrophoresis have been used to investigate the clonal diversity of T-cells in joint effusions from 16 patients with rheumatoid arthriti...

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Bibliographic Details
Published in:British journal of rheumatology 1995-03, Vol.34 (3), p.232-235
Main Authors: KARIM, S. N, MURPHY, E. A, STURROCK, R. D, GOUDIE, R. B
Format: Article
Language:English
Subjects:
Adult
Aged
Arthritis, Psoriatic - genetics
Arthritis, Psoriatic - immunology
Arthritis, Rheumatoid - genetics
Arthritis, Rheumatoid - immunology
Base Sequence
Biological and medical sciences
Cells, Cultured
Clone Cells
Diseases of the osteoarticular system
Female
Humans
Inflammatory joint diseases
Interleukin-2 - pharmacology
Lupus Erythematosus, Systemic - genetics
Lupus Erythematosus, Systemic - immunology
Male
Medical sciences
Middle Aged
Molecular Sequence Data
Synovial Fluid - cytology
Synovial Fluid - immunology
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
Time Factors
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Summary:T-cell receptor (TCR) gamma gene rearrangements which have been amplified in polymerase chain reactions (PCRs) and analysed by high resolution polyacrylamide gel electrophoresis have been used to investigate the clonal diversity of T-cells in joint effusions from 16 patients with rheumatoid arthritis, one with systemic lupus erythematosus and one with psoriatic arthropathy. Polyclonality was found in every case but an oligoclonal subset of dominant rearrangements was also demonstrated in all but the patient with psoriasis. Marked changes in the relative preponderance of the various clonotypes were observed in 29 of 48 paired tests from 12 cases before and after culture in media containing interleukin-2 (IL-2) showing that SF mononuclear cells cultured in vitro with IL-2 are not representative of those present in vivo.
ISSN:0263-7103
1460-2172