Acyl-CoA synthetase activity depends on the phospholipid composition of rat liver plasma membranes

The dependence of acyl-CoA synthetase on the lipid composition of rat liver plasma membranes has been investigated. For this purpose the composition of the membranes was modified by incorporation of different phospholipids in the presence of partially purified lipid transfer proteins. Another approa...

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Bibliographic Details
Published in:Journal of lipid mediators and cell signalling 1995, Vol.11 (1), p.13-23
Main Authors: Momchilova-Pankov, Albena B., Markovska, Tania T., Koumanov, Kamen S.
Format: Article
Language:English
Subjects:
Acyl-CoA synthetase
Animals
Cell Membrane - drug effects
Cell Membrane - enzymology
Coenzyme A Ligases - metabolism
d-Galactosamine
Galactosamine - pharmacology
Liver - enzymology
Liver - ultrastructure
Male
Phospholipase C
Phospholipids
Phospholipids - metabolism
Phospholipids - physiology
Plasma membranes
Rats
Rats, Wistar
Type C Phospholipases - metabolism
Type C Phospholipases - pharmacology
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Summary:The dependence of acyl-CoA synthetase on the lipid composition of rat liver plasma membranes has been investigated. For this purpose the composition of the membranes was modified by incorporation of different phospholipids in the presence of partially purified lipid transfer proteins. Another approach to the modification of the membrane phospholipid composition was treatment with exogenous phospholipase C and subsequent enrichment with different phospholipids. The experiments performed in vitro indicated that the presence of certain phospholipids such as phosphatidylnositol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine was essential for the activation of long chain fatty acids by aryl-CoA synthetase. However, some differences were observed when oleate and palmitate were used as substrates. Sphingomyelin was found to inhibit this activity especially when oleic acid served as substrate. In addition, we tried to modify in vivo the membrane lipid composition by treatment with n-galactosamine, which is known to induce acute hepatitis and cause biochemical and biophysical alterations in liver membranes. The results thus obtained confirmed the idea that the augmentation of the membrane lipids and especially of PI, PE and PG was accompanied by acyl-CoA synthetase activation. The presence of two different enzymes, activating the saturated and unsaturated fatty acids is discussed.
ISSN:0929-7855
DOI:10.1016/0929-7855(94)00024-7