Na,K-ATPase β subunit isoform expression in the peripheral nervous system of the rat

Using the RNase protection assay (RPA) to study the distribution of isoforms of the non-catalytic (β) subunit of Na,K-ATPase in the peripheral nervous system, we found both β1 and β2 isoform mRNAs in dorsal root ganglion (DRG), but only β2 mRNA in sciatic nerve. Using Western blot to measure accumul...

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Bibliographic Details
Published in:Neuroscience letters 1995-01, Vol.183 (3), p.206-209
Main Authors: Fink, David J., Fang, DaNan, Li, TieDong, Mata, Marina
Format: Article
Language:English
Subjects:
Animals
Axonal transport
Axonal Transport - physiology
Base Sequence
Blotting, Western
Densitometry
Dorsal root ganglion
Ganglia, Spinal - cytology
Ganglia, Spinal - enzymology
Isoenzymes - biosynthesis
Kinetics
Male
Molecular Sequence Data
Peripheral Nervous System - enzymology
Rats
Rats, Sprague-Dawley
RNA - biosynthesis
Schwann cell
Schwann Cells - enzymology
Sciatic nerve
Sciatic Nerve - enzymology
Sodium potassium transporting ATPase
Sodium-Potassium-Exchanging ATPase - biosynthesis
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Summary:Using the RNase protection assay (RPA) to study the distribution of isoforms of the non-catalytic (β) subunit of Na,K-ATPase in the peripheral nervous system, we found both β1 and β2 isoform mRNAs in dorsal root ganglion (DRG), but only β2 mRNA in sciatic nerve. Using Western blot to measure accumulation of the polypeptides at a ligature on the nerve we found that β1 but not β2 polypeptide is carried by rapid axonal transport in the sciatic nerve. These results imply that β1 is the prominent isoform of Na,K-ATPase in neurons and β2 the prominent isoform in Schwann cells.
ISSN:0304-3940
1872-7972
DOI:10.1016/0304-3940(94)11152-9