Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle

Four Boran cattle were infected with Trypanosoma brucei using Glossina morsitans centralis and were left untreated throughout the experimental period of 18 months. During this period, sequential blood samples were collected and examined for the presence of anti-trypanosome antibodies and their antig...

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Bibliographic Details
Published in:Veterinary parasitology 1995, Vol.56 (1), p.37-46
Main Authors: Masake, R.A., Moloo, S.K., Nantulya, V.M., Minja, S.H., Makau, J.M., Njuguna, J.T.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antibodies, Protozoan - blood
Antibody Formation
Antigenaemia
ANTIGENE
ANTIGENOS
ANTIGENS
Antigens, Protozoan - blood
BOVIN
CATTLE
Cattle-Protozoa
DIAGNOSIS
Diagnosis-Protozoa
DIAGNOSTIC
DIAGNOSTICO
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
GANADO BOVINO
Hematocrit
Male
Orchiectomy
Parasitemia - blood
Parasitemia - diagnosis
Parasitemia - immunology
Sensitivity and Specificity
TEST ELISA
Time Factors
TRYPANOSOMA BRUCEI
Trypanosoma brucei brucei - immunology
Trypanosoma brucei brucei - isolation & purification
Trypanosomiasis, Bovine - blood
Trypanosomiasis, Bovine - diagnosis
Trypanosomiasis, Bovine - immunology
Tsetse Flies - parasitology
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Summary:Four Boran cattle were infected with Trypanosoma brucei using Glossina morsitans centralis and were left untreated throughout the experimental period of 18 months. During this period, sequential blood samples were collected and examined for the presence of anti-trypanosome antibodies and their antigens. Using the buffy coat technique (BCT), trypanosomes were detected in 38 (16.3%) of the 233 blood samples. Unlike the BCT, antigen-detection enzyme-linked immunosorbent assay (Ag-ELISA) diagnosed infections in 189 (81.1%) of the blood samples. These results were supported by the presence of anti-trypanosome antibodies in the same samples. Thus Ag-ELISA was 5.5 times more sensitive than the BCT. Towards the end of the observation period, G.m. centralis tsetse were fed on the aparasitaemic cattle to determine whether they still harboured the infection as the persistent antigenaemia seemed to suggest. Bloodmeals from the four cattle were infective to tsetse, thus emphasising the importance of Ag-ELISA in diagnosis of sub-patent infections.
ISSN:0304-4017
1873-2550
DOI:10.1016/0304-4017(94)00667-2