PCNA-Immunoreactivity in the uterus of rats after treatment with the antiestrogen Tamoxifen

We used an antibody to the proliferating cell nuclear antigen (PCNA) to investigate the effect of the long-term administration of tamoxifen on proliferative activity in the uterus of mature rats. Untreated cycling and ovariectomized rats served as controls. The PCNA labelling indices (PI) and the mi...

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Bibliographic Details
Published in:Annals of anatomy 1995-03, Vol.177 (2), p.133-138
Main Authors: Rumpel, Elisabeth, Michna, Horst, Kühnel, Wolfgang
Format: Article
Language:English
Subjects:
Animals
Cell Nucleus - drug effects
Cell Nucleus - ultrastructure
Epithelial Cells
Epithelium - drug effects
Estrus - physiology
Female
Immunohistochemistry
Ovariectomy
PCNA immunohistochemistry
Proliferating Cell Nuclear Antigen - analysis
Proliferation
Rat uterus
Rats
Rats, Sprague-Dawley
Reference Values
Tamoxifen
Tamoxifen - pharmacology
Uterus - cytology
Uterus - drug effects
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Summary:We used an antibody to the proliferating cell nuclear antigen (PCNA) to investigate the effect of the long-term administration of tamoxifen on proliferative activity in the uterus of mature rats. Untreated cycling and ovariectomized rats served as controls. The PCNA labelling indices (PI) and the mitotic indices (MI) were estimated for the luminal and glandular epithelium and for the stromal fibroblasts. A strong correlation was found for PI and MI in the luminal and in the glandular epithelium, and a lower, but also significant correlation, for the endometrial stroma cells. Tamoxifen treatment decreased the PI of the luminal epithelial cells and of the stroma as much as ovariectomy. In both of these groups, the proportion of anti-PCNA positive cells in the glandular epithelium was significantly higher than in the luminal epithelium. These data indicate that tamoxifen has a strong antiproliferative effect on the uterus of mature rats, and that this antiestrogenic action is cell type specific.
ISSN:0940-9602
1618-0402
DOI:10.1016/S0940-9602(11)80060-9